用于高通量检测贮藏土豆软腐病和疫霉腐烂病的挥发性生物标志物的鉴定。

Identification of volatile biomarkers for high-throughput sensing of soft rot and Pythium leak diseases in stored potatoes.

机构信息

Department of Biological Systems Engineering, Washington State University, 1935 E. Grimes Way, PO Box 646120, Pullman, WA, USA.

Department of Horticulture, PO Box 646414, Washington State University, Pullman, WA, USA.

出版信息

Food Chem. 2022 Feb 15;370:130910. doi: 10.1016/j.foodchem.2021.130910. Epub 2021 Sep 4.

DOI:10.1016/j.foodchem.2021.130910

PMID:34788943

Abstract

Soft rot and Pythium leak are postharvest storage diseases of potato tubers that can cause substantial crop losses in the US. This study focused on detecting volatile organic compounds (VOCs) associated with rot inoculated tubers during storage (up to 21 days) using headspace solid-phase microextraction (SPME) coupled to gas chromatography (GC) with mass spectrometry (MS) and flame ionization detector (FID) analysis. Russet Burbank and Ranger Russet tubers were inoculated with the rot pathogens. Static sampling with 50 min trapping time followed by GC-MS and GC-FID analysis identified 23 and 30 common VOCs from the pathogen inoculated tubers. Overall, n,n-dimethylmethylamine, acetone, 1-undecene, and styrene, occurred frequently and repeatability in inoculated samples based on GC-MS analysis, with the latter two found using GC-FID analysis as well. Identification of such biomarkers can be useful in developing high-throughput VOC sensing systems for early disease detection in potato storage facilities.

摘要

软腐病和绵疫病是马铃薯块茎采后贮藏期的病害，在美国可导致大量作物损失。本研究采用顶空固相微萃取（SPME）结合气相色谱（GC）-质谱联用（MS）和火焰离子化检测器（FID）分析，聚焦于检测贮藏过程中（长达 21 天）接种腐烂马铃薯块茎的挥发性有机化合物（VOCs）。用软腐病菌和绵疫病菌接种俄亥俄州立大学 883 号和拉格朗日 883 号马铃薯块茎。静态采样 50 分钟，用 SPME 捕获，然后进行 GC-MS 和 GC-FID 分析，从接种病菌的块茎中鉴定出 23 种和 30 种常见 VOCs。总体而言，基于 GC-MS 分析，n,n-二甲基甲酰胺、丙酮、1-十一烯和苯乙烯在接种样品中频繁出现且重现性好，而 GC-FID 分析也发现了后两种物质。此类生物标志物的鉴定有助于开发用于早期检测马铃薯贮藏设施中病害的高通量 VOC 传感系统。