Department of Biomedical and Dental Sciences and MorphoFunctional Imaging, University of Messina, 98122 Messina, Italy.
Department of Clinical and Experimental Medicine, Unit of Infectious Diseases, University of Messina, 98122 Messina, Italy.
Int J Environ Res Public Health. 2021 Aug 26;18(17):8999. doi: 10.3390/ijerph18178999.
HIV-positive people showed a high oral prevalence of HPV-DNA and have a greater incidence of head and neck carcinomas compared to general population. We performed a molecular survey evaluating the presence of HPV-DNA in saliva of HIV-positive and HIV-negative subjects in order to quantify the risk represented by HIV-positivity. The sample was made up by 102 subjects: 40 HIV-positive, 32 HIV-negative with sexual risk behaviors (SRB) and 30 HIV-negative without risk factors. DNA was extracted from cellular pellets and HPV detection and genotyping were performed by PCR assays. In the HIV-positive group (of which 58.3% declared SRB) 33.33% of the sample were HPV-positive (33.33% to high-risk genotypes, 25.0% to low-risk genotypes and 41.66% to other genotypes). In the HIV-negative SRB group, HPV-positive subjects were 37.04% (60.0% to high risk genotypes, 20.0% to low risk genotypes, and 20.0% to other genotypes). Finally, in the control group, the HPV-positive subjects were 7.14% (50% to high-risk genotypes and 50% to low-risk genotypes). In the HIV group, concerning the HPV positivity, there was no significant difference between subjects with and without SRBs. In summary, we found a high oral HPV-DNA detection in HIV+ group, showing a strong relationship between HIV and HPV.
HIV 阳性者的 HPV-DNA 口腔患病率较高，与普通人群相比，头颈部癌的发病率更高。我们进行了一项分子调查，评估了 HIV 阳性和 HIV 阴性受试者唾液中 HPV-DNA 的存在情况，以量化 HIV 阳性的风险。该样本由 102 名受试者组成：40 名 HIV 阳性，32 名具有性风险行为（SRB）的 HIV 阴性和 30 名无危险因素的 HIV 阴性。从细胞沉淀中提取 DNA，并通过 PCR 检测进行 HPV 检测和基因分型。在 HIV 阳性组（其中 58.3% 报告有 SRB）中，33.33%的样本为 HPV 阳性（33.33%为高危基因型，25.0%为低危基因型，41.66%为其他基因型）。在 HIV 阴性 SRB 组中，HPV 阳性者为 37.04%（60.0%为高危基因型，20.0%为低危基因型，20.0%为其他基因型）。最后，在对照组中，HPV 阳性者为 7.14%（50%为高危基因型，50%为低危基因型）。在 HIV 组中，关于 HPV 阳性，有和没有 SRB 的受试者之间没有显著差异。总之，我们发现 HIV+组口腔 HPV-DNA 检测呈高阳性，表明 HIV 和 HPV 之间存在很强的关系。
