Division of Virology, National Public Health Institute, Budapest, Hungary.
Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, Szeged, Hungary.
Infect Genet Evol. 2018 Apr;59:99-106. doi: 10.1016/j.meegid.2018.01.030. Epub 2018 Feb 1.
In addition to traditional risk factors such as smoking, alcohol consumption and betel nut use, human papillomavirus (HPV) infection also plays a role in the development of head and neck squamous cell carcinomas (HNSCCs). Although among European countries the highest incidence and mortality rates of head and neck cancer types were recorded in Hungary, data regarding HPV prevalence in HNSCCs is scarce. We collected biopsy and saliva samples from patients diagnosed with HNSCC or oral potentially malignant disorders (OPMDs) and tested them for the presence of HPV using the PCR consensus primer set MY09/11 and the GP5+/6+ primer pair. HPV genotypes were assessed by sequencing of the amplified PCR fragments. Oral mucosa and saliva samples from tumor- and OPMD-free individuals were also analysed. HPV was detected in 11 out of 60 HNSCC samples (18%). All of the HPV positive tumors carried HPV type 16. 5 out of the 57 saliva samples collected from HNSCC patients was HPV positive (8.8%); among them, in addition to HPV16, HPV13 was also detected. Tumors located to the oropharynx had the highest HPV positivity rate with 50% (7 out of 14), which was significantly higher than the HPV prevalence in oral mucosa samples collected from controls (0 out of 20; p > 0.001) or in OPMD biopsies (0 out of 21, p > 0.001). 2 out of 57 control saliva samples (3.5%, subtype HPV13 and 11) and 3 out of 39 saliva samples from OPMD patients (7.7%, subtype HPV18, 81 and 10) were HPV positive. Our data suggested that HPV16 infection may contribute, in concert with cigarette smoking, to the development of a subset of head and neck cancers in Hungary. HPV16 infection per se does not account, however, for the high HNSCC incidence rate recorded in this country.
除了传统的危险因素，如吸烟、饮酒和嚼槟榔，人乳头瘤病毒（HPV）感染也在头颈部鳞状细胞癌（HNSCC）的发生发展中发挥作用。虽然在欧洲国家中，匈牙利的头颈部癌症发病率和死亡率最高，但有关 HNSCC 中 HPV 流行率的数据却很少。我们收集了诊断为 HNSCC 或口腔潜在恶性疾病（OPMD）患者的活检和唾液样本，并使用 PCR 通用引物 MY09/11 和 GP5+/6+引物对检测 HPV 的存在。通过扩增 PCR 片段的测序评估 HPV 基因型。还分析了来自无肿瘤和 OPMD 个体的口腔黏膜和唾液样本。在 60 个 HNSCC 样本中检测到 11 个（18%）HPV。所有 HPV 阳性肿瘤均携带 HPV 16 型。从 57 个 HNSCC 患者采集的唾液样本中，有 5 个（8.8%）为 HPV 阳性；其中，除 HPV16 外，还检测到 HPV13。HPV 阳性率最高的是口咽肿瘤，为 50%（14 个中的 7 个），显著高于对照组口腔黏膜样本（20 个中 0 个；p>0.001）或 OPMD 活检样本（21 个中 0 个；p>0.001）的 HPV 流行率。57 个对照唾液样本中有 2 个（3.5%，HPV13 和 11 亚型）和 39 个 OPMD 患者唾液样本中有 3 个（7.7%，HPV18、81 和 10 亚型）为 HPV 阳性。我们的数据表明，HPV16 感染可能与吸烟一起，促成匈牙利部分头颈部癌症的发生。然而，HPV16 感染本身并不能解释该国 HNSCC 发病率高的原因。
