Jardón-Maximino Noemi, Pérez-Alvarez Marissa, Cadenas-Pliego Gregorio, Lugo-Uribe Luis E, Cabello-Alvarado Christian, Mata-Padilla José M, Barriga-Castro Enrique Díaz
Centro de Investigación en Química Aplicada (CIQA), Saltillo 25294, Coahuila, Mexico.
Centro de Tecnología Avanzada CIATEQ, Lerma 52004, Estado de México, Mexico.
Polymers (Basel). 2021 Aug 25;13(17):2846. doi: 10.3390/polym13172846.
In this work, we report the synthesis of copper nanoparticles (Cu NPs), employing the chemical reduction method in an aqueous medium. We used copper sulfate pentahydrate (CuSO·5HO) as a metallic precursor; polyethylenimine (PEI), allylamine (AAM), and 4-aminobutyric acid (AABT) as stabilizing agents; and hydrated hydrazine as a reducing agent. The characterization of the obtained nanoparticles consisted of X-ray, TEM, FTIR, and TGA analyses. Through these techniques, it was possible to detect the presence of the used stabilizing agents on the surface of the NPs. Finally, a zeta potential analysis was performed to differentiate the stability of the nanoparticles with a different type of stabilizing agent, from which it was determined that the most stable nanoparticles were the Cu NPs synthesized in the presence of the PEI/AAM mixture. The antimicrobial activity of Cu/PEI/AABT toward and bacteria was high, inhibiting both bacteria with low contact times and copper concentrations of 50-200 ppm. The synthesis method allowed us to obtain Cu NPs free of oxides, stable to oxidation, and with high yields. The newly functionalized Cu NPs are potential candidates for antimicrobial applications.
在本工作中，我们报道了在水介质中采用化学还原法合成铜纳米颗粒（Cu NPs）。我们使用五水硫酸铜（CuSO₄·5H₂O）作为金属前驱体；聚乙烯亚胺（PEI）、烯丙胺（AAM）和4-氨基丁酸（AABT）作为稳定剂；水合肼作为还原剂。对所得纳米颗粒的表征包括X射线、透射电子显微镜（TEM）、傅里叶变换红外光谱（FTIR）和热重分析（TGA）。通过这些技术，可以检测到纳米颗粒表面存在所用的稳定剂。最后，进行了zeta电位分析以区分不同类型稳定剂的纳米颗粒的稳定性，由此确定最稳定的纳米颗粒是在PEI/AAM混合物存在下合成的Cu NPs。Cu/PEI/AABT对两种细菌的抗菌活性很高，在低接触时间和50 - 200 ppm的铜浓度下就能抑制这两种细菌。该合成方法使我们能够获得无氧化物、抗氧化且产率高的Cu NPs。新功能化的Cu NPs是抗菌应用的潜在候选材料。
