Department of Orthopedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa, 920-8641, Japan.
Sci Rep. 2021 Sep 9;11(1):18001. doi: 10.1038/s41598-021-97573-0.
Autologous cancellous bone (ACB) grafting is the "gold standard" treatment for delayed bone union. However, small animal models for such grafts are lacking. Here, we developed an ACB graft rat model. Anatomical information regarding the iliac structure was recorded from five rat cadavers (10 ilia). Additionally, 5 and 25 rats were used as controls and ACB graft models, respectively. A defect was created in rat femurs and filled with ACB. Post-graft neo-osteogenic potential was assessed by radiographic evaluation and histological analysis. Iliac bone harvesting yielded the maximum amount of cancellous bone with minimal invasiveness, considering the position of parailiac nerves and vessels. The mean volume of cancellous bone per rat separated from the cortical bone was 73.8 ± 5.5 mm. Bone union was evident in all ACB graft groups at 8 weeks, and new bone volume significantly increased every 2 weeks (P < 0.001). Histological analysis demonstrated the ability of ACB grafts to act as a scaffold and promote bone union in the defect. In conclusion, we established a stable rat model of ACB grafts by harvesting the iliac bone. This model can aid in investigating ACB grafts and development of novel therapies for bone injury.
自体松质骨（ACB）移植是治疗延迟性骨愈合的“金标准”治疗方法。然而，这种移植物的小动物模型是缺乏的。在这里，我们开发了一种 ACB 移植大鼠模型。从五具大鼠尸体（10 个髂骨）中记录了髂骨结构的解剖信息。此外，分别使用 5 只和 25 只大鼠作为对照和 ACB 移植模型。在大鼠股骨中创建缺陷并用 ACB 填充。通过放射学评估和组织学分析评估移植后的新成骨潜力。考虑到副髂神经和血管的位置，从皮质骨中分离髂骨可获得最大量的松质骨，且微创性最小。每只大鼠分离的皮质骨的松质骨体积平均为 73.8±5.5mm。所有 ACB 移植组在 8 周时均可见骨愈合，新骨体积每 2 周显著增加（P<0.001）。组织学分析表明，ACB 移植物能够作为支架促进缺损处的骨愈合。总之，我们通过采集髂骨建立了一种稳定的 ACB 移植大鼠模型。该模型可以帮助研究 ACB 移植物，并为骨损伤开发新的治疗方法。
