Li Wenxi, Li Jielin, Xu Ying, Huang Yan, Xu Shuqi, Ou Zirui, Long Xiaoli, Li Xinyu, Liu Xinyu, Xiao Zening, Huang Jiaqi, Chen Weizhao
College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, China.
College of Materials Science and Engineering, Shenzhen University, Shenzhen, 518060, China.
Synth Syst Biotechnol. 2021 Aug 24;6(3):216-223. doi: 10.1016/j.synbio.2021.08.002. eCollection 2021 Sep.
Gardenia blue is a natural blue pigment that is environmentally friendly, non-toxic, and stable. The hydrolysis of geniposide, catalyzed by β-glucosidase, is a critical step in the production process of gardenia blue. However, β-glucosidase is not resistant to high temperatures, limiting the production of gardenia blue. In this study, we investigated the effectiveness of a heat-resistant glucosidase obtained from in the production of gardenia blue. The enzyme exhibited a maximum activity of 10.60 U/mL at 90 °C. Single-factor and orthogonal analyses showed that exogenously expressed heat-resistant glucosidase reacted with 470.3 μg/mL geniposide and 13.5 μg/mL glycine at 94.2 °C, producing a maximum yield of 26.2857 μg/mL of gardenia blue after 156.6 min. When applied to the dyeing of denim, gardenia blue produced by this method yielded excellent results; the best color-fastness was achieved when an iron ion mordant was used. This study revealed the feasibility and application potential of microbial production of gardenia blue.
栀子蓝是一种天然蓝色素，具有环保、无毒且稳定的特点。在栀子蓝的生产过程中，β-葡萄糖苷酶催化栀子苷的水解是关键步骤。然而，β-葡萄糖苷酶不耐高温，这限制了栀子蓝的生产。在本研究中，我们考察了从[具体来源未提及]获得的耐热葡萄糖苷酶在栀子蓝生产中的效果。该酶在90°C时表现出最高活性为10.60 U/mL。单因素和正交分析表明，外源表达的耐热葡萄糖苷酶在94.2°C下与470.3 μg/mL栀子苷和13.5 μg/mL甘氨酸反应，156.6分钟后栀子蓝的最大产量为26.2857 μg/mL。将此方法生产的栀子蓝应用于牛仔布染色时，效果优异；使用铁离子媒染剂时可可可获得最佳色牢度。本研究揭示了微生物生产栀子蓝的可行性及应用潜力。
