Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres" (INGEBI-CONICET), Ciudad Autónoma de Buenos Aires, Buenos Aires, C1428ADN, Argentina.
STAR Protoc. 2021 Sep 1;2(3):100703. doi: 10.1016/j.xpro.2021.100703. eCollection 2021 Sep 17.
The pathogen differentiates from epimastigotes (E) into infective metacyclic trypomastigotes (MTs) to invade the mammalian cell. This process, called metacyclogenesis, is mimicked in vitro by nutrient starvation or incubation with minimal media. Here, we describe an alternative protocol for metacyclogenesis by incubating E forms in a biphasic medium supplemented with human blood. Although time consuming, this procedure yields fully differentiated MTs without the presence of intermediate forms, even for cultures that have been maintained as E for years.
病原体从滋养体（E）分化为感染性的循环体（MT），以侵入哺乳动物细胞。这个过程称为循环生成，在体外可以通过营养饥饿或在最小培养基中孵育来模拟。在这里，我们描述了一种通过在含有人血的双相培养基中孵育 E 形式来进行循环生成的替代方案。尽管此过程耗时较长，但即使对于已经维持多年的 E 培养物，也可以得到完全分化的 MT，而没有中间形式的存在。
