Graduate School of Tianjin Medical University, Tianjin Central Hospital of Obstetrics and Gynecology, Tianjin Key Laboratory of Human Development and Reproduction Regulation, Tianjin, 300070, China.
Graduate School of Tianjin Medical University, Tianjin Central Hospital of Obstetrics and Gynecology, Tianjin Key Laboratory of Human Development and Reproduction Regulation, Tianjin, 300070, China.
Taiwan J Obstet Gynecol. 2021 Sep;60(5):816-820. doi: 10.1016/j.tjog.2021.07.006.
This study was designed to evaluate the correlation between serum pentraxin-3 (PTX3)/hypersensitivity CRP (hs-CRP) expression and obesity during pregnancy and their application as inflammatory biomarkers in obese pregnant women.
Pregnant women scheduled to experience a single-birth at our hospital between 2016 and 2017 were selected for this nested case-control study. These patients were evaluated for age and gestational age in the first trimester (11-14 weeks), had their body mass index (BMI) calculated and were subjected to an OGTT between Week 24 and 28 of pregnancy. Obese patients with normal OGTT and a BMI of ≥30 kg/m in the second trimester were selected as the obese group (OBE, n = 80), and non-obese pregnant women with normal OGTT with a BMI of <30 kg/m were selected as the control group (CON, n = 80). ELISA was used to detect the expression of PTX3 and hs-CRP.
The expression of both PTX3 and hs-CRP increased in both groups, with increasing gestational age (P < 0.05). However, hs-CRP level in Group OBE was increased, compared to that in the healthy control (P < 0.01), during the second trimester. PTX3 expression was also significantly higher in OBE samples than in the control (P < 0.05), during the third trimester; correlation analysis demonstrated that PTX3 was positively correlated with hs-CRP, BMI, fasting plasma glucose and HOMA-IR.
The expression levels of both PTX3 and hs-CRP increased with increasing gestational age, and PTX3 expression was related to BMI, which serves to confirm the inflammatory response in these patients.
本研究旨在评估血清 pentraxin-3 (PTX3)/高敏 C 反应蛋白 (hs-CRP) 表达与妊娠期肥胖之间的相关性，并将其作为肥胖孕妇的炎症生物标志物。
选择 2016 年至 2017 年在我院单胎分娩的孕妇进行这项巢式病例对照研究。这些患者在孕早期（11-14 周）评估年龄和孕周，计算体重指数（BMI），并在妊娠 24-28 周进行 OGTT。选择孕中期 BMI≥30kg/m2且 OGTT 正常的肥胖患者为肥胖组（OBE，n=80），选择孕中期 BMI<30kg/m2且 OGTT 正常的非肥胖孕妇为对照组（CON，n=80）。采用 ELISA 法检测 PTX3 和 hs-CRP 的表达。
两组 PTX3 和 hs-CRP 的表达均随孕周增加而增加（P<0.05），但 OBE 组 hs-CRP 水平在孕中期高于健康对照组（P<0.01）。OBE 组 PTX3 表达在孕晚期也明显高于对照组（P<0.05）。相关性分析显示，PTX3 与 hs-CRP、BMI、空腹血糖和 HOMA-IR 呈正相关。
PTX3 和 hs-CRP 的表达水平随孕周增加而增加，PTX3 表达与 BMI 相关，证实了这些患者存在炎症反应。
