Pekonen F, Weintraub B D
Endocrinology. 1978 Nov;103(5):1668-77. doi: 10.1210/endo-103-5-1668.
The TSH-binding properties of human lymphocytes in continuous culture were studied and compared to those of bovine and human thyroid cells in primary culture. Both lymphocytes and thyroid cells had maximal TSH-binding capacity at pH 5.2. At pH 7.4, thyroid cells bound 15% but lymphocytes bound only 3% of the amount bound at pH 5.2. At 37 C, maximal binding of [125I]iodo-TSH to lymphocytes was reached within 60--90 min and maximal binding to thyroid cells was reached within 15--20 min. TSH binding to lymphocytes was salt sensitive, being inhibited to 50% by 0.2 mM MgCl and 0.4 mM CaCl2 and by 20 mM Kl, KCl, and NaCl. The saturable binding of bovine TSH (bTSH) to thyroid cells at pHs 5.2 and 7.4 was above 90% of the total binding. Saturable binding of bovine TSH (bTSH) to thyroid cells at pHs 5.2 and 7.4 was above 90% of the total binding. Saturable binding to lymphocytes at pH 5.2 was also above 90%, but at pH 7.4 was 75% of total. At pH 5.2, both cell types displayed identical displacement curves of [125I]iodo-bTSH by unlabeled bTSH. Pure hCG, human placental lactogen, human GH, and insulin cross-reacted to less than 1% with [125I]iodo-bTSH binding to lymphocytes at pH 5.2, whereas a crude preparation of hCG and human FSH plus human LH showed a strong cross-reaction. Nonhormone glycoproteins, including mucin, normal human gamma-globulin, and bovine thyroglobulin showed intermediate cross-reactivity. At pH 7.4, the cross-reactivity of normal human gamma-globulin, bovine thyroglobulin, and pure hCG with bTSH binding to both lymphocytes and thyroid cells was below 1%. The TSH-binding properties of lymphocytes and thyroid cells show many similarities but differ in kinetics and the relative binding capacity at neutral pH. Although the physiological significance of these differences is not yet clear, cultured cells provide a convenient system for studies of TSH-receptor interaction.
对连续培养的人淋巴细胞的促甲状腺激素（TSH）结合特性进行了研究，并与原代培养的牛和人甲状腺细胞的TSH结合特性进行了比较。淋巴细胞和甲状腺细胞在pH 5.2时均具有最大TSH结合能力。在pH 7.4时，甲状腺细胞结合的量为pH 5.2时结合量的15%，而淋巴细胞仅结合3%。在37℃时，[125I]碘-TSH与淋巴细胞的最大结合在60 - 90分钟内达到，与甲状腺细胞的最大结合在15 - 20分钟内达到。TSH与淋巴细胞的结合对盐敏感，0.2 mM MgCl₂和0.4 mM CaCl₂以及20 mM KI、KCl和NaCl可将其抑制至50%。在pH 5.2和7.4时，牛TSH（bTSH）与甲状腺细胞的可饱和结合占总结合的90%以上。在pH 5.2和7.4时，牛TSH（bTSH）与甲状腺细胞的可饱和结合占总结合的90%以上。在pH 5.2时，淋巴细胞的可饱和结合也超过90%，但在pH 7.4时为总量的75%。在pH 5.2时，两种细胞类型对未标记的bTSH显示出相同的[125I]碘-bTSH置换曲线。在pH 5.2时，纯人绒毛膜促性腺激素（hCG）、人胎盘催乳素、人生长激素（GH）和胰岛素与[125I]碘-bTSH与淋巴细胞的结合交叉反应率低于1%，而hCG粗制品以及人促卵泡激素（FSH）加人促黄体生成素（LH）则显示出强烈的交叉反应。非激素糖蛋白，包括粘蛋白、正常人γ球蛋白和牛甲状腺球蛋白表现出中等程度的交叉反应。在pH 7.4时，正常人γ球蛋白、牛甲状腺球蛋白和纯hCG与bTSH与淋巴细胞和甲状腺细胞的结合交叉反应率均低于1%。淋巴细胞和甲状腺细胞的TSH结合特性显示出许多相似之处，但在动力学以及中性pH下的相对结合能力方面存在差异。尽管这些差异的生理意义尚不清楚，但培养细胞为研究TSH受体相互作用提供了一个便利的系统。
