Coutelier J P, Kehrl J H, Bellur S S, Kohn L D, Notkins A L, Prabhakar B S
Laboratory of Oral Medicine, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892.
J Clin Immunol. 1990 Jul;10(4):204-10. doi: 10.1007/BF00918653.
The expression and functional relevance of thyroid stimulating hormone (TSH) receptors on human immune cells were studied. Flow cytometric analysis was used to study the binding of biotinylated TSH to human peripheral blood mononuclear cells (PBMC) and various purified lymphoid populations. Our results indicate that the hormone binds well to monocytes and natural killer (NK) cells and marginally to purified tonsillar T and B lymphocytes. There was a significant increase in the binding of TSH to purified B cells that were activated in vitro with Staphylococcus aureaus Cowan. In contrast, the binding of TSH to T cells was unaltered when they were stimulated with phytohemagglutinin (PHA). While TSH increases DNA synthesis and intracellular cAMP levels of FRTL-5 rat thyroid cells, it did not have such stimulatory effects on lymphocytes. However, there was a moderate increase in Ig production by activated B lymphocytes when they were cultured in the presence of the hormone. A possible function for TSH as a link between the immune system and the thyroid is discussed.
对人免疫细胞上促甲状腺激素（TSH）受体的表达及其功能相关性进行了研究。采用流式细胞术分析生物素化TSH与人外周血单个核细胞（PBMC）及各种纯化淋巴细胞群体的结合情况。我们的结果表明，该激素与单核细胞和自然杀伤（NK）细胞结合良好，与纯化的扁桃体T和B淋巴细胞结合较弱。用金黄色葡萄球菌考恩株体外激活的纯化B细胞，TSH与之结合显著增加。相反，用植物血凝素（PHA）刺激T细胞时，TSH与T细胞的结合未发生改变。虽然TSH可增加FRTL-5大鼠甲状腺细胞的DNA合成和细胞内cAMP水平，但对淋巴细胞没有这种刺激作用。然而，在有该激素存在的情况下培养时，活化的B淋巴细胞产生的Ig有适度增加。文中讨论了TSH作为免疫系统与甲状腺之间联系的一种可能功能。
