Azratul-Hizayu T, Chen C D, Lau K W, Azrizal-Wahid N, Tan T K, Lim Y A L, Sofian-Azirun M, Low V L
Institute for Advanced Studies (IAS), Universiti Malaya, 50603 Kuala Lumpur, Malaysia.
Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603 Kuala Lumpur, Malaysia.
Trop Biomed. 2021 Sep 1;38(3):327-337. doi: 10.47665/tb.38.3.073.
This study aims to examine the efficacy of mosquito mat vaporizers on Aedes aegypti and their associated metabolic detoxication mechanisms. For this purpose, Aedes aegypti (Linnaeus) was collected from nine districts in Selangor, Malaysia and tested with mosquito vaporizing mat bioassays. The same populations were also subjected to biochemical assays to investigate activities of detoxifying enzymes, namely non-specific esterase (EST), glutathione-S-transferase (GST) and mixed function oxidase (MFO). The efficacy of Ae. aegypti on the active ingredients tested in decreasing order were d- allethrin > dimefluthrin > prallethrin with PBO > prallethrin. The results further indicated significant enhancement mean levels of EST, GST and MFO in pyrethroid-resistant populations. The mortality rate of Ae. aegypti in response to pyrethroid active ingredients was associated with MFO activity, suggesting it is an important detoxification enzyme for the populations tested. In view of the presence of resistance against household insecticide products, pyrethroid efficacy on Ae. aegypti populations needs to be monitored closely to ensure the implementation of an effective vector control program in Malaysia.
本研究旨在检验电热蚊香片对埃及伊蚊的功效及其相关的代谢解毒机制。为此，从马来西亚雪兰莪的九个地区采集了埃及伊蚊（林奈），并用电热蚊香片生物测定法进行测试。对相同种群还进行了生化测定，以研究解毒酶的活性，即非特异性酯酶（EST）、谷胱甘肽 - S - 转移酶（GST）和混合功能氧化酶（MFO）。埃及伊蚊对所测试活性成分的功效从高到低依次为右旋烯丙菊酯> 氯氟醚菊酯> 炔丙菊酯（添加增效醚PBO）> 炔丙菊酯。结果进一步表明，在拟除虫菊酯抗性种群中，EST、GST和MFO的平均水平显著提高。埃及伊蚊对拟除虫菊酯活性成分的死亡率与MFO活性相关，表明它是所测试种群的一种重要解毒酶。鉴于对家用杀虫剂产品存在抗性，需要密切监测拟除虫菊酯对埃及伊蚊种群的功效，以确保在马来西亚实施有效的病媒控制计划。
