Cao Jifan, Zheng Wende, Chen Baizhong, Yan Zhenping, Tang Xiaowen, Li Jiahao, Zhang Zhen, Ang Song, Li Chen, Wu Rihui, Wu Panpan, Chen Wen-Hua
School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China.
International Healthcare Innovation Institute (Jiangmen), Jiangmen 529040, China.
Insects. 2024 May 11;15(5):345. doi: 10.3390/insects15050345.
The overuse of synthetic insecticides has led to various negative consequences, including insecticide resistance, environmental pollution, and harm to public health. This may be ameliorated by using insecticides derived from botanical sources. The primary objective of this study was to evaluate the anti-mosquito activity of the essential oil (EO) of Blanco cv. Chachiensis (Chachi) (referred to as CRB) at immature, semi-mature, and mature stages. The chemical compositions of the CRB EO were analyzed using GC-MS. The main components were identified to be D-limonene and -terpinene. The contents of D-limonene at the immature, semi-mature, and mature stages were 62.35%, 76.72%, and 73.15%, respectively; the corresponding contents of -terpinene were 14.26%, 11.04%, and 11.27%, respectively. In addition, the corresponding contents of a characteristic component, methyl 2-aminobenzoate, were 4.95%, 1.93%, and 2.15%, respectively. CRB EO exhibited significant larvicidal activity against (, Diptera: Culicidae), with the 50% lethal doses being 65.32, 61.47, and 65.91 mg/L for immature, semi-mature, and mature CRB EO, respectively. CRB EO was able to inhibit acetylcholinesterase and three detoxification enzymes, significantly reduce the diversity of internal microbiota in mosquitoes, and decrease the relative abundance of core species within the microbiota. The present results may provide novel insights into the utilization of plant-derived essential oils in anti-mosquitoes.
合成杀虫剂的过度使用已导致各种负面后果，包括抗药性、环境污染和对公众健康的危害。使用植物源杀虫剂可能会改善这种情况。本研究的主要目的是评估布兰科品种查奇恩西斯（Chachi）（简称CRB）精油在未成熟、半成熟和成熟阶段的抗蚊活性。使用气相色谱-质谱联用仪（GC-MS）分析了CRB精油的化学成分。主要成分被鉴定为D-柠檬烯和γ-萜品烯。未成熟、半成熟和成熟阶段D-柠檬烯的含量分别为62.35%、76.72%和73.15%；γ-萜品烯的相应含量分别为14.26%、11.04%和11.27%。此外，一种特征成分2-氨基苯甲酸甲酯的相应含量分别为4.95%、1.93%和2.15%。CRB精油对致倦库蚊（Culex quinquefasciatus，双翅目：蚊科）表现出显著的杀幼虫活性，未成熟、半成熟和成熟CRB精油的半数致死剂量分别为65.32、61.47和65.91mg/L。CRB精油能够抑制乙酰胆碱酯酶和三种解毒酶，显著降低蚊子体内微生物群的多样性，并降低微生物群中核心物种的相对丰度。目前的结果可能为植物源精油在抗蚊方面的应用提供新的见解。
