Shah S I A, Tipu M Y, Aslam A, Khan A U, Shafee M, Khan S A, Khan N U, Akbar A
Department of Pathology, University of Veterinary & Animal Sciences, Lahore, Pakistan.
Center for Advanced Studies in Vaccinology & Biotechnology (CASVAB) University of Balochistan, Quetta, Pakistan.
Trop Biomed. 2021 Sep 1;38(3):353-359. doi: 10.47665/tb.38.3.078.
Curcumin is a potent antimicrobial herb used traditionally as a spice in culinary. This study was designed to evaluate the antiviral effects of curcuma longa extract against H influenza virus. A total of 60 embryonated eggs were equally divided into 5 groups with 12 eggs in each group. Group 1 (G1) served as uninfected negative control. Whereas Group 2 (G2) was kept as positive control infected with known virus @ 0.2 ml with 10 EID. Group 3 (G3) was offered Curcuma longa @ 0.2 mg/0.2 ml and HN virus (@ 0.2 ml with 10 EID. Similarly, Group 4 (G4) was inoculated with extract of Curcuma longa @ 0.2 mg/0.2 ml per egg, whereas Group 5 (G5) was given Ribazole @ 0.2 ml/ egg. The crude extract and virus were administered on the 15 day of incubation and were checked after every 24 hours up to 96th hour post inoculation by random selection of 3 eggs. Death and survival rate were noted in all groups. Gross and histopathological lesions were also observed. Results revealed that Curcuma longa extract had significantly (p<0.05) reduced the mortality rate of embryos infected with HN virus. In G3, increased lymphocytes and mild fatty changes were seen in liver. Whereas, mature RBCs, plasma cells and some lymphoblast's were observed in Spleen. Similarly, the bursa follicles were with lymphocytic aggregation. The G4 (Curcuma longa) and G5 (Ribazole) were with maximum embryo survival after 48 and 72 h post inoculation. This study revealed potential antiviral activity of Curcuma longa against H9N2 influenza viruses and can be opted as alternative to antibiotics and antiviral drugs to minimize the antimicrobial resistance in human and animal population.
姜黄素是一种强效抗菌草药，传统上用作烹饪香料。本研究旨在评估姜黄提取物对甲型流感病毒的抗病毒作用。总共60枚受精鸡蛋被平均分为5组，每组12枚鸡蛋。第1组（G1）作为未感染的阴性对照。而第2组（G2）作为感染已知病毒的阳性对照，接种量为0.2 ml含10个鸡胚感染剂量（EID）。第3组（G3）给予姜黄0.2 mg/0.2 ml并接种甲型流感病毒（接种量为0.2 ml含10个EID）。同样，第4组（G4）每枚鸡蛋接种0.2 mg/0.2 ml的姜黄提取物，而第5组（G5）每枚鸡蛋给予利巴韦林0.2 ml。粗提取物和病毒在孵化第[X]天给药，接种后每24小时随机选取3枚鸡蛋进行检查，直至接种后96小时。记录所有组的死亡和存活率。还观察了大体和组织病理学病变。结果显示，姜黄提取物显著（p<0.05）降低了感染甲型流感病毒的胚胎死亡率。在G3组中，肝脏可见淋巴细胞增多和轻度脂肪变性。而在脾脏中观察到成熟红细胞、浆细胞和一些淋巴母细胞。同样，法氏囊滤泡有淋巴细胞聚集。G4组（姜黄）和G5组（利巴韦林）在接种后48小时和72小时胚胎存活率最高。本研究揭示了姜黄对H9N2流感病毒具有潜在的抗病毒活性，可作为抗生素和抗病毒药物的替代品，以尽量减少人和动物群体中的抗菌药物耐药性。 （注：原文中“15 day of incubation”未明确是第15天还是15天内，这里按“第15天”翻译，可能需要根据实际情况调整）
