Rasool Amir, Khan Muti-Ur-Rehman, Ali Muhammad Asad, Anjum Aftab Ahmad, Ahmed Ishtiaq, Aslam Asim, Mustafa Ghulam, Masood Saima, Ali Muhammad Amjad, Nawaz Muhammad
Department of Pathology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Pak J Pharm Sci. 2017 Jul;30(4):1341-1344.
In the present study, anti-Avian influenza virus H9N2 activity of aqueous extracts (5, 10, 15, 20, 25%) of Zingiber officinalis and Allium sativum was evaluated. Embryo-toxicity was evaluated by histopathological scoring of Chorio-allantoic membrane of chick embryos. Cytotoxicity of extracts was determined by MTT assay on Vero cells. Aqueous extract of ginger had antiviral activity at 10, 15, 20 and 25% while garlic had activity at 15, 20 and 25%. Histopathological scoring of chorio-allantoic membrane for aqueous extracts (5, 10, 15, 20, 25%) of ginger (0.66±0.57, 1.33±0.57, 1.66±0.57, 2.66±0.57, 3.66±0.57, respectively) and garlic (1.00±0.00, 1.33±0.57, 2.00±0.00, 2.33±0.57, 3.66±0.57, respectively) was concentration dependant. MTT assay revealed cytotoxicity of both plants was also concentration dependent. Extracts of ginger (5, 10, 15, 20, 25%) had lower cytotoxicity (71, 59, 28, 22, 0 % cell survival, respectively) as compared to garlic (61, 36. 20, 11, 3% cell survival, respectively). Overall results revealed that concentration of aqueous extract of ginger (10%), showing antiviral activity against HN, was less toxic to vero cells (> 50% cell survival). It is insinuated that ginger may have anti- Avian influenza virus HN potential and its active compounds needs further investigations.
在本研究中，评估了姜（Zingiber officinalis）和大蒜（Allium sativum）水提取物（5%、10%、15%、20%、25%）对甲型禽流感病毒H9N2的活性。通过对鸡胚绒毛尿囊膜进行组织病理学评分来评估胚胎毒性。采用MTT法测定提取物对Vero细胞的细胞毒性。生姜水提取物在10%、15%、20%和25%浓度时有抗病毒活性，而大蒜在15%、20%和25%浓度时有活性。生姜（5%、10%、15%、20%、25%）和大蒜（5%、10%、15%、20%、25%）水提取物对绒毛尿囊膜的组织病理学评分呈浓度依赖性。MTT法显示，两种植物提取物的细胞毒性也呈浓度依赖性。与大蒜（细胞存活率分别为61%、36%、20%、11%、3%）相比，生姜（5%、10%、15%、20%、25%）提取物的细胞毒性较低（细胞存活率分别为71%、59%、28%、22%、0%）。总体结果表明，对H9N2显示出抗病毒活性的生姜水提取物（10%）对Vero细胞的毒性较小（细胞存活率>50%）。由此推测，生姜可能具有抗甲型禽流感病毒H9N2的潜力，其活性成分有待进一步研究。
