College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei, 430023, PR China.
College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei, 430023, PR China.
Microb Pathog. 2021 Nov;160:105169. doi: 10.1016/j.micpath.2021.105169. Epub 2021 Sep 10.
Vibrio parahaemolyticus is one of the most common pathogenic bacteria that pose a threat to human health. The purpose of this study was to investigate antibacterial mechanisms of ε-poly-lysine (ε-PL) against V. parahaemolyticus using a lable free-based proteomic analysis. The differentially expressed proteins (DEPs) were subjected to bioinformatics analysis. The results indicated that a total of 196 DEPs, including 118 up-regulated and 78 down-regulated, were identified in the ε-PL-treated cells compared with control group. Upon Go functional enrichment, 13, 9, and 8 specific Go terms in biological processes, molecular functions and cellular components were identified, respectively. KEGG pathways analysis indicated that the DEPs were mainly involved in bacterial chemotaxis, RNA transport and two-component system, which were significantly enriched (P < 0.05). In PPI analysis, Che R and Che V, both involved in bacterial chemotaxis and RNA transport pathways, are closely related to other DEPs. Therefore, the down-regulation of Che R and Che V in ε-PL-treated cells resulted in the reduction or even loss of bacterial adaptability, and they were the critical action sites of ε-PL to inactivate V. parahaemolyticus.
副溶血弧菌是威胁人类健康的最常见的病原菌之一。本研究旨在采用无标记蛋白质组学分析方法研究 ε-聚赖氨酸（ε-PL）对副溶血弧菌的抗菌机制。对差异表达蛋白（DEPs）进行生物信息学分析。结果表明，与对照组相比，ε-PL 处理组细胞中鉴定出 196 个 DEP，包括 118 个上调和 78 个下调。GO 功能富集分析显示，在生物学过程、分子功能和细胞组成中分别有 13、9 和 8 个特定的 GO 术语。KEGG 通路分析表明，DEPs 主要参与细菌趋化作用、RNA 转运和双组分系统，这些通路显著富集（P<0.05）。在 PPI 分析中，参与细菌趋化作用和 RNA 转运途径的 Che R 和 Che V 与其他 DEPs 密切相关。因此，ε-PL 处理细胞中 Che R 和 Che V 的下调导致细菌适应性降低甚至丧失，它们是 ε-PL 灭活副溶血弧菌的关键作用位点。
