College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei, P.R. China.
School of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, Hubei, P.R. China.
J Appl Microbiol. 2021 Aug;131(2):791-800. doi: 10.1111/jam.14954. Epub 2021 Jan 4.
The purpose of this study was to investigate the regulatory mechanism of ε-PL on Shewanella putrefaciens.
Proteomics analysis of inhibitory effect of ε-PL against S. putrefaciens was performed by label-free quantitative assay based on high-resolution mass spectrometry (MS). Quantification of 2206 proteins was obtained with high confidence, and a total of 36 differentially expressed proteins (DEPs), with 10 and 26 proteins showing upregulation and downregulation, respectively, were identified. Upon Go functional enrichment, 11, 5 and 8 specific Go terms in biological processes, molecular functions and cellular components were identified, respectively. Six KEGG pathways, including 'ribosome', were significantly enriched. Among the ribosome pathway, there were seven DEPs and all of them were distributed on large and small subunits of ribosome.
The significant downregulation of proteins, large subunits of ribosomal proteins RP-L18, L30 and L27, small subunits ribosomal proteins S16 and S20, and RNA polymerase β' subunit protein rpoC were the critical action sites of ε-PL to inhibit S. putrefaciens growth.
Shewanella putrefaciens is one of the representative fish-spoilage bacteria regardless of fish type, and poses significant problems for the fish brewery. A better understanding of the antibacterial mechanism of ε-PL on S. putrefaciens could make important contributions to development of biological control strategies of these economically important pathogens.
本研究旨在探讨 ε-PL 对腐败希瓦氏菌的调控机制。
通过基于高分辨率质谱（MS）的无标记定量分析，对 ε-PL 抑制腐败希瓦氏菌的抑制作用进行蛋白质组学分析。高置信度获得了 2206 种蛋白质的定量结果，共鉴定出 36 种差异表达蛋白（DEPs），分别有 10 种和 26 种蛋白上调和下调。GO 功能富集后，分别在生物过程、分子功能和细胞成分中确定了 11、5 和 8 个特定的 GO 术语。有 6 个 KEGG 途径（包括“核糖体”）显著富集。在核糖体途径中，有 7 个 DEPs，它们都分布在核糖体的大亚基和小亚基上。
蛋白质的显著下调、核糖体蛋白 RP-L18、L30 和 L27 的大亚基、核糖体小亚基蛋白 S16 和 S20、RNA 聚合酶 β'亚基蛋白 rpoC 的下调是 ε-PL 抑制腐败希瓦氏菌生长的关键作用部位。
腐败希瓦氏菌是无论鱼类种类如何，都是代表性的鱼类腐败菌之一，对鱼类酿造厂构成了重大问题。更好地了解 ε-PL 对腐败希瓦氏菌的抗菌机制，将为开发这些经济重要病原体的生物防治策略做出重要贡献。
