Zhong Qingping, Wang Bin, Wang Jie, Liu Yufei, Fang Xiang, Liao Zhenlin
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, China.
SCAU (Chaozhou) Food Institute Co. Ltd., Chaozhou, China.
Front Microbiol. 2019 May 8;10:1045. doi: 10.3389/fmicb.2019.01045. eCollection 2019.
is a common pathogen which has become a major concern of seafood products. The bacteria in the viable but non-culturable (VBNC) state are unable to form colonies on growth media, but under appropriate conditions they can regain culturability. In this study, was induced into VBNC state at low temperature and oligotrophic condition, and was resuscitated to culturable state. The aim of this study is to explore the comparative proteomic profiles of the resuscitation state compared with the VBNC state and the exponential phase of using isobaric tags for relative and absolute quantitation (iTRAQ) technique. The differentially expressed proteins (DEPs) were subjected to GO functional annotations and KEGG pathway analysis. The results indicated that a total of 429 proteins were identified as the significant DEPs in the resuscitation cells compared with the VBNC cells, including 330 up-regulated and 99 down-regulated DEPs. Meanwhile, the resuscitation cells displayed 25 up-regulated and 36 down-regulated DEPs (total of 61 DEPs) in comparison with the exponential phase cells. The remarkable DEPs including ribosomal proteins, ABC transporters, outer membrane proteins and flagellar proteins. GO annotation showed that the 429 DEPs were classified into 37 GO terms, of which 17 biological process (BP) terms, 9 cellular component (CC) terms and 11 molecular function (MF) terms. The up-regulated proteins presented in all GO terms except two terms of developmental process and reproduction. The 61 DEPs were assigned to 23 GO terms, the up- and down-regulated DEPs were both mainly involved in cellular process, establishment of localization, metabolic process and so on. KEGG pathway analysis revealed that the 429 DEPs were assigned to 35 KEGG pathways, and the pathways of ribosome, glyoxylate and dicarboxylate metabolism were significantly enriched. Moreover, the 61 DEPs located in 26 KEGG pathways, including the significantly enriched KEGG pathways of ABC transporters and two-component system. This study would contribute to a better understanding of the molecular mechanism underlying the resuscitation of the VBNC state of .
是一种常见病原体，已成为海产品的主要关注点。处于活的但不可培养（VBNC）状态的细菌无法在生长培养基上形成菌落，但在适当条件下它们可以恢复可培养性。在本研究中，在低温和贫营养条件下被诱导进入VBNC状态，并复苏至可培养状态。本研究的目的是使用相对和绝对定量等压标签（iTRAQ）技术，探索复苏状态与VBNC状态以及的指数期相比的比较蛋白质组学图谱。对差异表达蛋白（DEP）进行了GO功能注释和KEGG通路分析。结果表明，与VBNC细胞相比，复苏细胞中共有429种蛋白被鉴定为显著DEP，包括330种上调和99种下调的DEP。同时，与指数期细胞相比，复苏细胞显示出25种上调和36种下调的DEP（共61种DEP）。显著的DEP包括核糖体蛋白、ABC转运蛋白、外膜蛋白和鞭毛蛋白。GO注释表明，429种DEP被分为37个GO术语，其中17个生物过程（BP）术语、9个细胞成分（CC）术语和11个分子功能（MF）术语。上调蛋白出现在除发育过程和繁殖这两个术语之外的所有GO术语中。61种DEP被分配到23个GO术语中，上调和下调的DEP都主要参与细胞过程、定位建立、代谢过程等。KEGG通路分析表明，429种DEP被分配到35条KEGG通路中，核糖体、乙醛酸和二羧酸代谢通路显著富集。此外，61种DEP位于26条KEGG通路中，包括ABC转运蛋白和双组分系统的显著富集的KEGG通路。本研究将有助于更好地理解的VBNC状态复苏的分子机制。
