Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Fredriksberg C, Denmark.
Bisgaard Consulting, Viby Sj, Denmark.
Vet Microbiol. 2021 Nov;262:109232. doi: 10.1016/j.vetmic.2021.109232. Epub 2021 Sep 6.
The aim of the investigation was to predict the serotypes of M. haemolytica based on whole genomic sequences with the capsular gene region as target. A total of 22 strains selected to have been serotyped and to represent all serotypes were investigated by whole genomic sequencing. The BIGSdb (Bacterial Isolate Genome Sequence Database) was downloaded and installed on a Linux server. Here the sequence database was setup with unique loci at serotype level. The server allows serotypes of M. haemolytica to be predicted from whole genomic sequences and the service is available to the public for free from https://ivsmlst.sund.root.ku.dk.
本研究旨在通过以荚膜基因区域为目标的全基因组序列来预测 M. haemolytica 的血清型。选择了总共 22 株已被血清分型并代表所有血清型的菌株进行全基因组测序。下载并在 Linux 服务器上安装了 BIGSdb（细菌分离基因组序列数据库）。在这里，在血清型水平上设置了唯一的基因座序列数据库。该服务器允许根据全基因组序列预测 M. haemolytica 的血清型，并且可以从 https://ivsmlst.sund.root.ku.dk 免费为公众提供该服务。
