Department of Internal Medicine, Veterinary Faculty, Aydın Adnan Menderes University, 09100, Aydın, Turkey.
Department of Biochemistry, Veterinary Faculty, Aydın Adnan Menderes University, 09100, Aydın, Turkey.
Vet Parasitol. 2021 Nov;299:109574. doi: 10.1016/j.vetpar.2021.109574. Epub 2021 Sep 9.
This study was designed to test the prophylactic and therapeutic efficacy of clinoptilolite against Cryptosporidium (C.) parvum infection in lambs. Within the first day of life, three groups of 10 lambs were each inoculated with 1 × 10 oocysts of C. parvum. The prophylactic (PROP) group received orally clinoptilolite supplemented at a rate of 3% to the colostrum within the first day of life before inoculation and then to milk replacer for two weeks. The therapeutic (TREA) group was supplemented with the same rate, route and duration of clinoptilolite, starting from the day of the first appearance of oocysts in the faeces. The positive control group (pCON) was left untreated and fed only the basal diet. Disease development and clinoptilolite efficacy were assessed daily by evaluating oocyst per gram of faeces (OPG) counts, faecal consistent score (FCS), and clinical health score (CHS) from day -1 to 20 days post inoculation. A significantly (p < 0.001) lower OPG value was found in the PROP and TREA groups in comparison to the pCON group. The FCS and CHS were decreased in the PROP (p < 0.01 and p < 0.001) and TREA (p < 0.05 and p < 0.001) groups compared to the pCON group, respectively. The percentage efficacy of clinoptilolite was calculated to be 97.4 % in the PROP group and 91.6 % in the TREA group. In conclusion, this study proved for the first time that clinoptilolite has promising prophylactic and therapeutic activities against C. parvum in experimentally infected lambs.
本研究旨在测试斜发沸石对羔羊感染小隐孢子虫（C. parvum）的预防和治疗效果。在出生后的第一天，三组 10 只羔羊分别接种 1×10 个小隐孢子虫卵囊。预防组（PROP）在接种前的第一天通过口服补充 3%的斜发沸石到初乳中，然后用代乳料补充两周。治疗组（TREA）以相同的比例、途径和时间用斜发沸石补充，从粪便中首次出现卵囊的那一天开始。阳性对照组（pCON）未进行治疗，仅喂食基础日粮。从接种前一天到第 20 天，通过评估每克粪便中的卵囊数（OPG）、粪便一致性评分（FCS）和临床健康评分（CHS）来评估疾病发展和斜发沸石的疗效。与 pCON 组相比，PROP 和 TREA 组的 OPG 值显著（p < 0.001）降低。与 pCON 组相比，PROP 组（p < 0.01 和 p < 0.001）和 TREA 组（p < 0.05 和 p < 0.001）的 FCS 和 CHS 降低。斜发沸石的疗效百分比在 PROP 组中计算为 97.4%，在 TREA 组中计算为 91.6%。综上所述，本研究首次证明斜发沸石对实验感染羔羊的小隐孢子虫具有有前途的预防和治疗作用。
