从山羊奶中分离出的念珠菌属的特性、酶活性及生物膜形成
Characterization, enzymatic activity and biofilm formation of Candida species isolated from goat milk.
作者信息
Segundo Zaragoza Carolina, López Ortiz Itzel, Contreras Caro Del Castillo David Alejandro, Domínguez Hernández Yesmín María, Rodríguez García Juan Antonio
机构信息
Laboratorio de Micología Veterinaria, Unidad de Servicios de Diagnóstico y Constatación, Centro de Enseñanza, Investigación y Extensión en Producción Animal en Altiplano, Facultad de Medicina Veterinaria y Zootecnia, UNAM, Mexico.
Laboratorio de Micología Veterinaria, Unidad de Servicios de Diagnóstico y Constatación, Centro de Enseñanza, Investigación y Extensión en Producción Animal en Altiplano, Facultad de Medicina Veterinaria y Zootecnia, UNAM, Mexico.
出版信息
Rev Iberoam Micol. 2021 Oct-Dec;38(4):175-179. doi: 10.1016/j.riam.2021.06.001. Epub 2021 Sep 10.
BACKGROUND
Data regarding yeast microbiota in goat milk is scarce.
AIMS
To isolate and identify species of the genus Candida in milk samples from clinically healthy goats, and evaluate their enzymatic activity and biofilm formation.
METHODS
1092 milk samples from clinically healthy goats were collected and processed. The yeast isolates were identified by phenotypic, methods and their enzymatic activity (phospholipase, hemolysin and protease) and biofilm formation evaluated.
RESULTS
We obtained 221 Candida isolates belonging to six species: Candida kefyr (35.7%), Candida guilliermondii (33%), Candida famata (23.5%), Candida glabrata (5.9%), Candida albicans (1.35%) and Candida parapsilosissensu lato (0.45%). Protease activity was detected in all Candida species while hemolysin activity was only present in C. kefyr, C. guilliermondii, C. famata and C. albicans. Only C. albicans showed phospholipase activity. With the exception of C. parapsilosis sensu lato, all Candida species formed biofilm, with 60.19% of the isolates being poor producers, 9.93% moderate producers, and 1.35% strong producers.
CONCLUSIONS
The milk of clinically healthy goats contains several species of the genus Candida that could play a role as opportunistic pathogens in mastitis.
背景
关于山羊奶中酵母微生物群的数据稀缺。
目的
从临床健康山羊的奶样中分离和鉴定念珠菌属的菌种，并评估它们的酶活性和生物膜形成能力。
方法
收集并处理了1092份来自临床健康山羊的奶样。通过表型方法鉴定酵母分离株，并评估其酶活性（磷脂酶、溶血素和蛋白酶）以及生物膜形成能力。
结果
我们获得了221株念珠菌分离株，属于六个菌种：凯菲念珠菌（35.7%）、季也蒙念珠菌（33%）、法塔念珠菌（23.5%）、光滑念珠菌（5.9%）、白色念珠菌（1.35%）和近平滑念珠菌复合群（0.45%）。在所有念珠菌菌种中均检测到蛋白酶活性，而溶血素活性仅在凯菲念珠菌、季也蒙念珠菌、法塔念珠菌和白色念珠菌中存在。只有白色念珠菌显示出磷脂酶活性。除近平滑念珠菌复合群外，所有念珠菌菌种均形成生物膜，其中60.19%的分离株生物膜形成能力较差，9.93%为中等，1.35%为较强。
结论
临床健康山羊的奶中含有几种念珠菌属菌种，它们可能在乳腺炎中作为机会性病原体发挥作用。
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