Chen Buze, Jin Xin, Wang Haihong, Zhou Qingmei, Li Guilin, Lu Xiaoyuan
Department of Gynecology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China.
Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People's Republic of China.
Int J Gen Med. 2021 Sep 3;14:5193-5201. doi: 10.2147/IJGM.S327673. eCollection 2021.
The present study aims to explore the expression, clinical significance, and prospective pathway signaling of miR-501-3p in ovarian cancer (OC) based on database and informatics analysis.
Kruskal-Wallis test, Wilcoxon sign-rank test, and logistic regression were used to evaluate the relationship between clinical features and miR-501-3p expression. Kaplan-Meier survival curve analysis was used to explore the relationship between miR-501-3p expression and the prognosis of OC patients. The miRNA targets were obtained from databases TargetScan, miRanda, TarBase, miRTarBase, miR2Disease, miRecords, and miRWalk. GO and KEGG analyses were used to analyze the significant involvement of miR-501-3p target genes in function.
The low miR-501-3p expression in OC was significantly associated with histologic grade (P=0.015). Low miR-501-3p expression predicted a poorer overall survival (HR: 0.77; 95% CI: 0.61-0.96; P=0.02) and disease-specific survival (HR: 0.77; 95% CI: 0.61-0.99; P=0.038). GO and KEGG analyses demonstrated that miR-501-3p might participate in the development of OC by pathways including one carbon pool by folate, protein digestion and absorption, cell cycle, kaposi sarcoma-associated herpesvirus infection, and viral carcinogenesis.
Low miR-501-3p expression is significantly associated with poor survival in OC patients. It may be a promising prognostic biomarker for OC patients.
本研究旨在基于数据库和信息学分析，探讨miR-501-3p在卵巢癌（OC）中的表达、临床意义及潜在的信号通路。
采用Kruskal-Wallis检验、Wilcoxon符号秩检验和逻辑回归评估临床特征与miR-501-3p表达之间的关系。使用Kaplan-Meier生存曲线分析探讨miR-501-3p表达与OC患者预后的关系。从TargetScan、miRanda、TarBase、miRTarBase、miR2Disease、miRecords和miRWalk数据库中获取miRNA靶点。使用GO和KEGG分析来分析miR-501-3p靶基因在功能上的显著参与情况。
OC中miR-501-3p低表达与组织学分级显著相关（P=0.015）。miR-501-3p低表达预示着较差的总生存期（HR：0.77；95%CI：0.61-0.96；P=0.02）和疾病特异性生存期（HR：0.77；95%CI：0.61-0.99；P=0.038）。GO和KEGG分析表明，miR-501-3p可能通过包括叶酸一碳池、蛋白质消化与吸收、细胞周期、卡波西肉瘤相关疱疹病毒感染和病毒致癌作用等途径参与OC的发生发展。
miR-5
