大肠杆菌酪氨酸tRNA su3编码基因中的一个ρ依赖性终止位点。
A rho-dependent termination site in the gene coding for tyrosine tRNA su3 of Escherichia coli.
作者信息
Küpper H, Sekiya T, Rosenberg M, Egan J, Landy A
出版信息
Nature. 1978 Mar 30;272(5652):423-8. doi: 10.1038/272423a0.
Abstract
A set of partially overlapping DNA restriction fragments that support promoter-dependent transcription of the tRNATyr1 gene of Escherichia coli has been used to study site-specific termination in vitro. Transcription termination occurs at a specific site 224-226 nucleotides beyond the end of the structural gene and is completely dependent on rho-factor. Certain features of this site suggest differences from other termination sites previously studied. A role for specific sequence recognition is suggested.
摘要
一组部分重叠的DNA限制性片段被用于体外研究位点特异性终止，这些片段支持大肠杆菌tRNATyr1基因的启动子依赖性转录。转录终止发生在结构基因末端下游224 - 226个核苷酸的特定位点，并且完全依赖于rho因子。该位点的某些特征表明与先前研究的其他终止位点存在差异。这表明了特定序列识别的作用。
相似文献
1
A rho-dependent termination site in the gene coding for tyrosine tRNA su3 of Escherichia coli.大肠杆菌酪氨酸tRNA su3编码基因中的一个ρ依赖性终止位点。
Nature. 1978 Mar 30;272(5652):423-8. doi: 10.1038/272423a0.
2
Site-specific mutagenesis on cloned DNAs: generation of a mutant of Escherichia coli tyrosine suppressor tRNA in which the sequence G-T-T-C corresponding to the universal G-T-pseudouracil-C sequence of tRNAs is changed to G-A-T-C.克隆DNA的位点特异性诱变：大肠杆菌酪氨酸抑制性tRNA突变体的产生，其中对应于tRNA通用G-T-假尿嘧啶-C序列的G-T-T-C序列被改变为G-A-T-C。
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4753-7. doi: 10.1073/pnas.78.8.4753.
3
The tyrT locus: termination and processing of a complex transcript.tyrT基因座：一个复杂转录本的终止与加工
Cell. 1981 Nov;26(3 Pt 1):305-14. doi: 10.1016/0092-8674(81)90199-9.
4
Autogenous regulation of the gene for transcription termination factor rho in Escherichia coli: localization and function of its attenuators.大肠杆菌中转录终止因子rho基因的自体调控：其弱化子的定位与功能
J Bacteriol. 1986 Jun;166(3):945-58. doi: 10.1128/jb.166.3.945-958.1986.
5
Initiation and termination signals for transcription in bacteriophage M13.噬菌体M13转录的起始和终止信号
Nucleic Acids Res. 1984 May 25;12(10):4071-81. doi: 10.1093/nar/12.10.4071.
6
Rho-dependent transcription termination in the tyrT operon of Escherichia coli.大肠杆菌tyrT操纵子中的ρ因子依赖性转录终止
Gene. 1989;76(2):271-80. doi: 10.1016/0378-1119(89)90167-4.
7
Simultaneous gain and loss of functions caused by a single amino acid substitution in the beta subunit of Escherichia coli RNA polymerase: suppression of nusA and rho mutations and conditional lethality.大肠杆菌RNA聚合酶β亚基中单个氨基酸取代导致的功能同时获得与丧失：nusA和rho突变的抑制及条件致死性
Genetics. 1992 Mar;130(3):411-28. doi: 10.1093/genetics/130.3.411.
8
Chemical synthesis and cloning of a tyrosine tRNA gene.
Methods Enzymol. 1979;68:109-51. doi: 10.1016/0076-6879(79)68010-2.
9
The nucleotide sequence of the rho gene of E. coli K-12.大肠杆菌K-12的rho基因的核苷酸序列。
Nucleic Acids Res. 1983 Jun 11;11(11):3531-45. doi: 10.1093/nar/11.11.3531.
10
Effects of altered rho gene product on the expression of the Escherichia coli histidine operon.rho基因产物改变对大肠杆菌组氨酸操纵子表达的影响。
J Bacteriol. 1978 Dec;136(3):1201-4. doi: 10.1128/jb.136.3.1201-1204.1978.
引用本文的文献
1
In vivo regulation of bacterial Rho-dependent transcription termination by the nascent RNA.在活体内通过新生 RNA 对细菌 Rho 依赖性转录终止的调控。
J Biol Chem. 2022 Jun;298(6):102001. doi: 10.1016/j.jbc.2022.102001. Epub 2022 Apr 29.
2
Long Non-Coding RNAs in the Regulation of Gene Expression: Physiology and Disease.长链非编码RNA在基因表达调控中的作用：生理学与疾病
Noncoding RNA. 2019 Feb 17;5(1):17. doi: 10.3390/ncrna5010017.
3
Gene regulation of mammalian long non-coding RNA.哺乳动物长链非编码RNA的基因调控
Mol Genet Genomics. 2018 Feb;293(1):1-15. doi: 10.1007/s00438-017-1370-9. Epub 2017 Sep 11.
4
Rho directs widespread termination of intragenic and stable RNA transcription.Rho蛋白指导基因内和稳定RNA转录的广泛终止。
Proc Natl Acad Sci U S A. 2009 Sep 8;106(36):15406-11. doi: 10.1073/pnas.0903846106. Epub 2009 Aug 20.
5
Effects of bicyclomycin on RNA- and ATP-binding activities of transcription termination factor Rho.双环霉素对转录终止因子Rho的RNA结合活性和ATP结合活性的影响。
Antimicrob Agents Chemother. 1998 Mar;42(3):571-8. doi: 10.1128/AAC.42.3.571.
6
The tyrT locus of Escherichia coli B.大肠杆菌B的tyrT基因座
J Bacteriol. 1996 Apr;178(8):2469-70. doi: 10.1128/jb.178.8.2469-2470.1996.
7
Senescence-specific mitochondrial DNA molecules in P. anserina: evidence for transcription and normal processing of the RNA.鹅膏蕈（P. anserina）中衰老特异性线粒体DNA分子：RNA转录和正常加工的证据
Curr Genet. 1994 Jun;25(6):538-44. doi: 10.1007/BF00351675.
8
Linkage map of Escherichia coli K-12, edition 6.大肠杆菌K-12连锁图谱，第6版。
Microbiol Rev. 1980 Mar;44(1):1-56. doi: 10.1128/mr.44.1.1-56.1980.
9
Transcription in bacteriophage f1-infected Escherichia coli: RNA synthesized on DNA of deletion mutant PII shows the existence of a two-site terminator.噬菌体f1感染的大肠杆菌中的转录：在缺失突变体PII的DNA上合成的RNA显示存在一个双位点终止子。
Mol Gen Genet. 1984;195(3):411-7. doi: 10.1007/BF00341441.
10
A comprehensive sequence analysis program for the IBM personal computer.一款适用于IBM个人计算机的综合序列分析程序。
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):581-99. doi: 10.1093/nar/12.1part2.581.
本文引用的文献
1
Isolation and sequence determination of the 3'-terminal regions of isotopically labelled RNA molecules.同位素标记RNA分子3'末端区域的分离与序列测定
Nucleic Acids Res. 1974 May;1(5):653-71. doi: 10.1093/nar/1.5.653.
2
A two-dimensional fractionation procedure for radioactive nucleotides.一种用于放射性核苷酸的二维分级分离程序。
J Mol Biol. 1965 Sep;13(2):373-98. doi: 10.1016/s0022-2836(65)80104-8.
3
In vitro synthesis of tRNA Tyr precursors and their conversion to 4S RNA.酪氨酸转运核糖核酸前体的体外合成及其向4S核糖核酸的转化。
Nat New Biol. 1971 Sep 15;234(50):198-201. doi: 10.1038/newbio234198a0.
4
Tyrosine tRNA precursor molecule polynucleotide sequence.酪氨酸转运核糖核酸前体分子多核苷酸序列。
Nat New Biol. 1971 Sep 8;233(36):35-9. doi: 10.1038/newbio233035a0.
5
Nucleotide sequence of a ribonucleic acid transcribed in vitro from lambda phage deoxyribonucleic acid.从λ噬菌体脱氧核糖核酸体外转录的核糖核酸的核苷酸序列。
J Biol Chem. 1971 Aug 25;246(16):5120-39.
6
Duplicate genes for tyrosine transfer RNA in Escherichia coli.大肠杆菌中酪氨酸转运RNA的重复基因。
J Mol Biol. 1970 Jan 14;47(1):1-13. doi: 10.1016/0022-2836(70)90397-9.
7
Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose.32P 标记的寡核苷酸在二乙氨基乙基纤维素薄层上的色谱分析。
Eur J Biochem. 1969 Dec;11(2):395-9. doi: 10.1111/j.1432-1033.1969.tb00786.x.
8
Termination factor for RNA synthesis.RNA合成的终止因子。
Nature. 1969 Dec 20;224(5225):1168-74. doi: 10.1038/2241168a0.
9
Factor stimulating transcription by RNA polymerase.刺激RNA聚合酶转录的因子。
Nature. 1969 Jan 4;221(5175):43-6. doi: 10.1038/221043a0.
10
Transcriptional control of in vitro tRNA-Tyr synthesis.体外酪氨酸转运核糖核酸（tRNA-Tyr）合成的转录调控
Biochemistry. 1974 Sep 10;13(19):4058-62. doi: 10.1021/bi00716a038.
Zotero 插件