高血压女性中 Arntl mRNA 表达下调：一项病例对照研究。
Downregulation of Arntl mRNA Expression in Women with Hypertension: A Case-Control Study.
机构信息
Department of Epidemiology, School of Public Health, and Institute of Chronic Disease Prevention and Control, Wannan Medical College, Wuhu, China.
Anhui College of Traditional Chinese Medicine, Wuhu, China.
出版信息
Kidney Blood Press Res. 2021;46(6):741-748. doi: 10.1159/000518669. Epub 2021 Sep 3.
BACKGROUND
Previous studies have reported that disturbance of endogenous circadian rhythms enhances the chance of hypertension and suggested that circadian clock genes could have a crucial function in the onset of the disease. This case-control study was aimed to investigate the association of the mRNA expression of aryl hydrocarbon receptor nuclear translocator like (Arntl), clock circadian regulator (Clock), and period circadian regulators 1 and 2 (Per1 and Per2) with hypertension and blood pressure levels.
METHODS
A total of 172 subjects were recruited in this study, including 86 hypertension and 86 nonhypertension controls. The mRNA expression levels in peripheral blood mononuclear cells were determined by real-time quantitative polymerase chain reaction. The differences in Arntl, Clock, Per1, and Per2 mRNA expression were compared between the 2 groups, and the relationship between mRNA expression and cardiometabolic risk profiles was also assessed.
RESULTS
We found that the mRNA expression of Arntl was downregulated in the hypertension cases compared with controls in women (1.10 [0.66, 1.71] vs. 1.30 [0.99, 2.06], p = 0.031). There was a significant negative correlation between the Arntl mRNA expression and SBP (r = -0.301, p = 0.004) and DBP (r = -0.222, p = 0.034) in women. In men, a negative correlation between the Per1 mRNA expression and SBP (r = -0.247, p = 0.026) was found.
CONCLUSIONS
The Arntl mRNA expression may play an important role in progression of hypertension in women.
背景
先前的研究报告表明，内源性昼夜节律紊乱会增加患高血压的几率，并表明昼夜节律钟基因在疾病的发生中可能具有关键作用。本病例对照研究旨在探讨芳香烃受体核转位蛋白样（Arntl）、时钟节律调节因子（Clock）和周期节律调节因子 1 和 2（Per1 和 Per2）的 mRNA 表达与高血压和血压水平的关系。
方法
本研究共招募了 172 名受试者，包括 86 名高血压患者和 86 名非高血压对照者。通过实时定量聚合酶链反应测定外周血单个核细胞中的 mRNA 表达水平。比较了两组间 Arntl、Clock、Per1 和 Per2 mRNA 表达的差异，并评估了 mRNA 表达与心脏代谢风险谱之间的关系。
结果
我们发现，与对照组相比，女性高血压患者的 Arntl mRNA 表达下调（1.10 [0.66, 1.71] 与 1.30 [0.99, 2.06]，p = 0.031）。Arntl mRNA 表达与女性收缩压（r = -0.301，p = 0.004）和舒张压（r = -0.222，p = 0.034）呈显著负相关。在男性中，Per1 mRNA 表达与收缩压（r = -0.247，p = 0.026）呈负相关。
结论
Arntl mRNA 表达可能在女性高血压的进展中起重要作用。
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