National Laboratory of Solid State Microstructures, Collaborative Innovation Center of Advanced Microstructures, Chemistry and Biomedicine Innovation Center, College of Engineering and Applied Sciences, Jiangsu Key Laboratory of Artificial Functional Materials, Nanjing University, Nanjing, 210093, China.
West China School of Medicine, West China Hospital, Sichuan University, Chengdu, 610064, China.
Talanta. 2021 Dec 1;235:122799. doi: 10.1016/j.talanta.2021.122799. Epub 2021 Aug 13.
An accurate measurement of sweat glucose is a promising alternative to invasive finger prick blood test, and may provide effective self-monitoring of blood glucose with good patient compliance. Herein, an interrelated catalytic enzyme system has been developed, termed as CataFlower, which is composed of nanoflower MoS (peroxidase) decorated with GOx (glucose oxidase) and MnO (oxygen generator), and exhibits synergistic oxidative capability for sensitively monitoring sweat glucose. CataFlower can not only generate oxygen in situ to maximize GOx activity, but promote peroxidase-triggered HO oxidation of methylene blue, resulting in sensitive colorimetric detection of glucose. We identify that CataFlower can precisely detect glucose with a detection limit of 10 μM, allowing for measuring glucose levels in different biological samples, such as blood and urine. Particularly, CataFlower is capable of monitoring dynamic changes in sweat glucose with high sensitivity and accuracy during exercise. Therefore, CataFlower provides a stepping stone to eliminate invasive blood tests, significantly improving the diagnosis and management of diabetes mellitus.
准确测量汗液中的葡萄糖是一种有前途的替代方法，可以替代侵入性的手指采血检测，并且可以通过良好的患者依从性有效监测血糖。在这里，开发了一种相互关联的催化酶系统，称为 CataFlower，它由纳米花 MoS（过氧化物酶）组成，表面修饰有 GOx（葡萄糖氧化酶）和 MnO（氧气发生器），表现出协同氧化能力，可灵敏监测汗液中的葡萄糖。CataFlower 不仅可以原位产生氧气来最大程度地提高 GOx 的活性，还可以促进过氧化物酶引发的亚甲基蓝的 HO 氧化，从而实现对葡萄糖的灵敏比色检测。我们确定 CataFlower 可以精确检测 10μM 的葡萄糖，能够测量不同生物样本（如血液和尿液）中的葡萄糖水平。特别地，CataFlower 能够在运动过程中以高灵敏度和准确性监测汗液中葡萄糖的动态变化。因此，CataFlower 为消除侵入性血液检测提供了一个契机，显著改善了糖尿病的诊断和管理。
