Wang Zejun, Li Hongjun, Wang Jinqiang, Chen Zhaowei, Chen Guojun, Wen Di, Chan Amanda, Gu Zhen
Department of Bioengineering, University of California, Los Angeles, CA, 90095, United States; California NanoSystems Institute, University of California, Los Angeles, CA, 90095, United States.
Department of Bioengineering, University of California, Los Angeles, CA, 90095, United States; California NanoSystems Institute, University of California, Los Angeles, CA, 90095, United States; Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA, 90095, United States; Center for Minimally Invasive Therapeutics, University of California, Los Angeles, CA, 90095, United States.
Biomaterials. 2020 Apr;237:119782. doi: 10.1016/j.biomaterials.2020.119782. Epub 2020 Jan 13.
The integration of sampling and instant metabolite readout can fundamentally elevate patient compliance. To circumvent the need for complex in-lab apparatus, here, an all-in-one sampling and display transdermal colorimetric microneedle patch was developed for sensing hyperglycemia in mice. The coloration of 3,3',5,5'-tetramethylbenzidine (TMB) is triggered by the cascade enzymatic reactions of glucose oxidase (GOx) and horseradish peroxidase (HRP) at abnormally high glucose levels. The HRP in the upper layer is biomineralized with calcium phosphate (CaP) shell to add a pH responsive feature for increased sensitivity as well as protection from nonspecific reactions. This colorimetric sensor achieved minimally invasive extraction of the interstitial fluid from mice and converted glucose level to a visible color change promptly. Quantitative red green and blue (RGB) information could be obtained through a scanned image of the microneedle. This costless, portable colorimetric sensor could potentially detect daily glucose levels without blood drawing procedures.
采样与即时代谢物读数的整合能够从根本上提高患者的依从性。为了避免使用复杂的实验室设备，本文开发了一种一体化采样与显示的比色微针贴片，用于检测小鼠的高血糖情况。在异常高血糖水平下，葡萄糖氧化酶（GOx）和辣根过氧化物酶（HRP）的级联酶促反应会引发3,3',5,5'-四甲基联苯胺（TMB）的显色。上层的HRP用磷酸钙（CaP）壳进行生物矿化，以增加pH响应特性，提高灵敏度并防止非特异性反应。这种比色传感器实现了从小鼠体内微创提取组织间液，并迅速将葡萄糖水平转化为可见的颜色变化。通过微针的扫描图像可以获得定量的红、绿、蓝（RGB）信息。这种低成本、便携式的比色传感器有可能在无需采血程序的情况下检测每日血糖水平。
