Kim Eun Kyung, Kim Hye Min, Koo Ja Seung
Department of Pathology, National Health Insurance Service Ilsan Hospital, Goyang, Republic of Korea.
Department of Pathology, Yonsei University College of Medicine, Seoul, Republic of Korea.
Pathobiology. 2021;88(6):424-433. doi: 10.1159/000518208. Epub 2021 Sep 9.
The aim of this study was to investigate the expression patterns of glucose metabolism-related proteins and their clinicopathologic implications in adrenal cortical neoplasms (ACN) and pheochromocytoma (PCC).
Immunohistochemical staining was performed to evaluate glucose metabolism-related proteins (GLUT1, CAIX, hexokinase II, G6PDH, PHGDH, and SHMT1) in 132 ACN cases (115 adrenal cortical adenoma [ACA] and 17 adrenal cortical carcinoma [ACC]) and 189 PCC cases.
Expression levels of GLUT1 in tumor cells ([T]; p < 0.001), GLUT1 in stromal cells ([S]; p < 0.001), G6PDH (p < 0.001), and SHMT1 (p = 0.002) were higher in ACN than in PCC. GLUT1 (T; p = 0.045) and PHGDH (p = 0.043) levels were higher in ACC than in ACA. In a univariate analysis of ACN, GLUT1 (T; p = 0.017), CAIX (S; p = 0.003), and PHGDH (p = 0.009) levels were correlated with a shorter overall survival (OS). GLUT1 (T; p = 0.001) and PHGDH (p < 0.001) were related to a shorter OS in PCC. GLUT1 (T) positivity (p = 0.043) in ACN predicted a poor OS in a multivariate Cox analysis. In PCC, high GAPP score (p = 0.026), GLUT1 (T; p = 0.002), and PHGDH (p < 0.001) were independent prognostic factors for poor OS.
The adrenal gland tumors ACN and PCC had different expression patterns of glucose metabolism-related proteins (GLUT1, G6PDH, and SHMT1), with higher expression levels in ACN than in PCC. GLUT1 and PHGDH were significant prognostic factors in these adrenal neoplasms.
本研究旨在探讨肾上腺皮质肿瘤（ACN）和嗜铬细胞瘤（PCC）中葡萄糖代谢相关蛋白的表达模式及其临床病理意义。
采用免疫组织化学染色法评估132例ACN病例（115例肾上腺皮质腺瘤[ACA]和17例肾上腺皮质癌[ACC]）及189例PCC病例中葡萄糖代谢相关蛋白（GLUT1、CAIX、己糖激酶II、G6PDH、PHGDH和SHMT1）的表达情况。
ACN中肿瘤细胞（[T]；p<0.001）、基质细胞（[S]；p<0.001）中的GLUT1、G6PDH（p<0.001）和SHMT1（p=0.002）的表达水平高于PCC。ACC中GLUT1（T；p=0.045）和PHGDH（p=0.043）的水平高于ACA。在ACN的单因素分析中，GLUT1（T；p=0.017）、CAIX（S；p=0.003）和PHGDH（p=0.009）水平与总生存期（OS）较短相关。GLUT1（T；p=0.001）和PHGDH（p<0.001）与PCC中较短的OS相关。在多因素Cox分析中，ACN中GLUT1（T）阳性（p=0.043）预示OS较差。在PCC中，高GAPP评分（p=0.026）、GLUT1（T；p=0.002）和PHGDH（p<0.001）是OS较差的独立预后因素。
肾上腺肿瘤ACN和PCC具有不同的葡萄糖代谢相关蛋白（GLUT1、G6PDH和SHMT1）表达模式，ACN中的表达水平高于PCC。GLUT1和PHGDH是这些肾上腺肿瘤的重要预后因素。
