Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Social Security Organization, 17th Shahrivar Hospital, Mashhad, Iran.
Metab Brain Dis. 2021 Dec;36(8):2505-2510. doi: 10.1007/s11011-021-00841-1. Epub 2021 Sep 14.
Ischemic stroke is a devastating central nervous disease. Despite extensive research in to this area, few innovative neuroprotective treatments have been presented. 7-methoxycoumarin, also known as herniarin, is a common natural coumarin in several plant species. This project examined the effects of the herniarin in rats subjected to the middle cerebral artery occlusion (MCAO). Herniarin at doses of 10 and 20 mg/kg was administered through intraperitoneal injection for 7 days before MCAO induction. Rats were subjected to a 30 min MCAO and a subsequent 24 h' reperfusion. 24 h after the termination of MCAO, neurologic outcome, volume of brain infarction, level of interleukin-1β (IL-1β) and tumor necrosis factor alpha (TNF-α), as inflammatory markers, and oxidative stress markers including levels of total thiol, malondialdehyde (MDA), and superoxide dismutase (SOD) activity were estimated. Herniarin administration decreased the MCAO-induced infarct volume and neurological deficits. Moreover, pretreatment with herniarin significantly decreased the levels of MDA while simultaneously increasing the level of total thiol and SOD activity in the brain tissues of MCAO rats. Moreover, herniarin pretreatment decreased the levels of IL-1β and TNF-α in the brain tissues of MCAO rats. These results suggest that herniarin presents beneficial effects against ischemic stroke, partly through the inhibition of oxidative stress and inflammation.
缺血性脑卒中是一种严重的中枢神经系统疾病。尽管在这一领域进行了广泛的研究，但很少有创新的神经保护治疗方法被提出。7-甲氧基香豆素，也称为茴芹素，是几种植物中常见的天然香豆素。本项目研究了茴芹素对大脑中动脉闭塞（MCAO）大鼠的影响。在 MCAO 诱导前，通过腹腔注射给予茴芹素 10 和 20mg/kg 的剂量，连续 7 天。大鼠接受 30 分钟的 MCAO 和随后 24 小时的再灌注。MCAO 终止后 24 小时，评估神经功能结果、脑梗死体积、白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）水平，作为炎症标志物，以及总巯基、丙二醛（MDA）和超氧化物歧化酶（SOD）活性等氧化应激标志物的水平。茴芹素给药可减少 MCAO 引起的梗死体积和神经功能缺损。此外，茴芹素预处理显著降低了 MDA 的水平，同时增加了 MCAO 大鼠脑组织中总巯基和 SOD 活性的水平。此外，茴芹素预处理降低了 MCAO 大鼠脑组织中 IL-1β和 TNF-α的水平。这些结果表明，茴芹素对缺血性脑卒中具有有益的作用，部分是通过抑制氧化应激和炎症。
