前列腺组织中JPX和LINC00641非编码RNA的表达：一项病例对照研究。
JPX and LINC00641 ncRNAs expression in prostate tissue: a case-control study.
作者信息
Sajjadi Roshanak S, Modarressi Mohammad Hossein, Tabatabaiefar Mohammad Amin
机构信息
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, I.R. Iran.
出版信息
Res Pharm Sci. 2021 Aug 19;16(5):493-504. doi: 10.4103/1735-5362.323916. eCollection 2021 Oct.
BACKGROUND AND PURPOSE
Prostate cancer (PC) is the second most prevalent cancer in men. Prostate-specific antigen (PSA) is the main biomarker for screening PC. An increase in PSA could lead to false-positive results. Thus, more appropriate markers should be investigated. In the present study, JPX and LINC00641 expression levels were measured in tumoral prostate tissue compared with the non-tumor tissue.
EXPERIMENTAL APPROACH
43 pairs of prostate tumoral and non-tumor tissue were prepared. The expression levels of JPX and LINC00641 were investigated by RT-qPCR.
FINDINGS/RESULTS: Significant upregulation of LINC00641 (2.47 ± 0.5 1.41 ± 0.2) and downregulation of JPX (1.42 ± 0.6 2.83 ± 1.0) were observed in PC tissues compared with the normal tissues (their adjacent non-tumoral tissues).
CONCLUSION AND IMPLICATIONS
Dysregulation of JPX and LINC00641 in PC patients could be used in the future as a prognostic biomarker in PC.
背景与目的
前列腺癌（PC）是男性中第二常见的癌症。前列腺特异性抗原（PSA）是筛查PC的主要生物标志物。PSA升高可能导致假阳性结果。因此，应研究更合适的标志物。在本研究中，测定了肿瘤性前列腺组织与非肿瘤组织中JPX和LINC00641的表达水平。
实验方法
制备了43对前列腺肿瘤组织和非肿瘤组织。通过RT-qPCR研究JPX和LINC00641的表达水平。
研究结果
与正常组织（其相邻的非肿瘤组织）相比，在PC组织中观察到LINC00641显著上调（2.47±0.5对1.41±0.2），JPX显著下调（1.42±0.6对2.83±1.0）。
结论与意义
PC患者中JPX和LINC00641的失调未来可作为PC的预后生物标志物。
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