Clarke R N, Sexton T J, Ottinger M A
Poult Sci. 1982 Sep;61(9):1912-7. doi: 10.3382/ps.0611912.
A series of experiments was conducted to measure the respiratory activity, motility, and fertility of chicken and turkey sperm in undiluted and diluted (1 part semen to 5 parts Beltsville Poultry Semen Extender) semen held at either 41, 25, 15, or 5 C for 3 or 6 hr. Sperm respiration was temperature dependent, increasing with increasing temperature. When incubated under identical conditions, the respiratory rate of spermatozoa in diluted semen of both species was significantly (P less than .05) higher than in undiluted semen. In undiluted and diluted chicken and turkey semen, sperm mortality was lowest at 41 C. No differences in the motility of spermatozoa from undiluted and diluted semen of either species were observed in the unstored controls. Sperm motility in samples held at 15 or 5 C was similar to that of the unstored controls. The fertility of diluted chicken semen was highest (75%) after 6 hr of storage at 5 C. Fertility levels (71 to 83%) of chicken semen was highest (75%) after 6 hr of storage at 5 C. Fertility levels (71 to 83%) of chicken semen held for 3 hr at 25, 15, or 5 C did not differ from the unstored control. In contrast, the fertilizing capacity of diluted turkey semen (0 to 45%) was significantly lower than the unstored controls (68%), regardless of holding times and temperatures. The fertility of undiluted turkey semen was significantly (P less than .05) lower than the unstored control in all cases, with the exception of semen held at 25 C for 3 hr.
进行了一系列实验，以测量在41、25、15或5摄氏度下保存3小时或6小时的未稀释和稀释（精液与贝尔茨维尔家禽精液稀释剂按1:5比例）的鸡和火鸡精液中精子的呼吸活性、活力和受精能力。精子呼吸依赖于温度，随温度升高而增加。在相同条件下孵育时，两种物种稀释精液中精子的呼吸速率显著高于未稀释精液（P小于0.05）。在未稀释和稀释的鸡和火鸡精液中，精子死亡率在41摄氏度时最低。在未保存的对照中，未观察到两种物种未稀释和稀释精液中精子活力的差异。保存在15或5摄氏度的样本中精子活力与未保存的对照相似。稀释的鸡精液在5摄氏度下保存6小时后受精能力最高（75%）。在25、15或5摄氏度下保存3小时的鸡精液受精水平（71%至83%）与未保存的对照无差异。相比之下，无论保存时间和温度如何，稀释的火鸡精液的受精能力（0%至45%）显著低于未保存的对照（68%）。除了在25摄氏度下保存3小时的精液外，未稀释的火鸡精液在所有情况下的受精能力均显著低于未保存的对照（P小于0.05）。
