Mangena Phetole
Department of Biodiversity, School of Molecular and Life Sciences, Faculty of Science and Agriculture, University of Limpopo, Private Bag X1106, Sovenga, 0727, South Africa.
Heliyon. 2021 Sep 1;7(9):e07913. doi: 10.1016/j.heliyon.2021.e07913. eCollection 2021 Sep.
The physical and physiological roles of seed properties are often neglected during plant tissue culture. These properties determine the level of activity and performance of seeds, which is commonly known as seed vigour. This paper reports on the role of seed vigour on seed germination and shoot induction using cotyledonary node explants. For this, explants prepared from soybean seedlings established using seeds stored under ambient conditions for different durations (0, 3, 6 and 9-months) were used. The findings indicated that seed germination was highly instantaneous after harvest and began to decrease as seed storage was prolonged for 3, 6 and 9-months, respectively. Similar observations were made during shoot induction. Generally, the analysis revealed a positive relationship between seed vigour, germination and multiple shoot initiation as indicated by the Pearson's correlation coefficient reported. According to the findings, seed vigour could serve as a major obstacle to efficient germination and shoot proliferation for subsequent plant regeneration.
在植物组织培养过程中，种子特性的物理和生理作用常常被忽视。这些特性决定了种子的活性水平和表现，这通常被称为种子活力。本文报道了种子活力对使用子叶节外植体进行种子萌发和芽诱导的作用。为此，使用了从以不同时长（0、3、6和9个月）在环境条件下储存的种子培育出的大豆幼苗制备的外植体。研究结果表明，收获后种子萌发非常迅速，随着种子分别储存3、6和9个月，萌发率开始下降。在芽诱导过程中也有类似的观察结果。总体而言，分析表明，如所报道的皮尔逊相关系数所示，种子活力、萌发和多芽起始之间存在正相关关系。根据研究结果，种子活力可能是后续植物再生过程中高效萌发和芽增殖的主要障碍。
