Amado Paula Avelar, Castro Ana Hortência Fonsêca, Azevedo Lucas Santos, Aguilar Mariana Guerra de, Pimenta Lúcia Pinheiro Santos, Lima Luciana Alves Rodrigues Dos Santos
Campus Centro-Oeste Dona Lindu, Universidade Federal de São João Del-Rei (UFSJ), Divinópolis 35501-296, MG, Brazil.
Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil.
Plants (Basel). 2025 May 3;14(9):1383. doi: 10.3390/plants14091383.
This study aimed to assess the production of seedlings in an in vitro environment and their adaptation to natural conditions, as well as the callus induction, the chemical profile of calli extracts, and their antioxidant potential. The seedlings were obtained from seeds germinated in Murashige and Skoog (MS) medium. The germination rate was 33%, and about 22% of the seeds produced whole seedlings. Three-month-old seedlings were acclimatized for two months, resulting in an 80% survival rate and improved physiological characteristics. Callus induction was initiated from leaf explants obtained from seedlings and plant growth regulators (PGRs), with and without light exposure. Calli extracts were obtained using methanol; phenolic compound and flavonoid quantification were performed, and the chemical profile was determined by nuclear magnetic resonance (H NMR). For comparison, methanol extract from leaves collected in Brazilian Cerrado were also analyzed. Antioxidant activity was assessed using the 2,2-diphenyl-1-picryl-hydrazyl method and the ferric-reducing antioxidant power assay. All samples exhibited antioxidant activity according to the methods employed. Furthermore, H NMR revealed metabolic profile changes in the calli extracts compared to the leaf extract. This study yielded promising results, suggesting that in vitro culture could improve productivity and conserve the species, although changes were observed in the metabolic profile of .
本研究旨在评估体外环境下幼苗的生产及其对自然条件的适应性，以及愈伤组织诱导、愈伤组织提取物的化学特征及其抗氧化潜力。幼苗取自于在Murashige和Skoog（MS）培养基中发芽的种子。发芽率为33%，约22%的种子长出了完整的幼苗。三个月大的幼苗驯化了两个月，成活率达80%，生理特性也有所改善。从幼苗和植物生长调节剂（PGR）获得的叶片外植体开始进行愈伤组织诱导，有无光照均可。使用甲醇获得愈伤组织提取物；进行酚类化合物和黄酮类化合物的定量分析，并通过核磁共振（H NMR）确定化学特征。为作比较，还分析了从巴西塞拉多采集的叶片的甲醇提取物。使用2,2-二苯基-1-苦基肼法和铁还原抗氧化能力测定法评估抗氧化活性。根据所采用的方法，所有样品均表现出抗氧化活性。此外，与叶片提取物相比，H NMR显示愈伤组织提取物的代谢特征发生了变化。本研究取得了有前景的结果，表明尽管愈伤组织提取物的代谢特征有变化，但体外培养可以提高生产力并保护该物种。
