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LuxR 家族转录调节因子 abaR 在鲍曼不动杆菌生物膜形成中的作用机制。

Mechanism Underlying the Role of LuxR Family Transcriptional Regulator abaR in Biofilm Formation by Acinetobacter baumannii.

机构信息

Department of Burn, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

出版信息

Curr Microbiol. 2021 Nov;78(11):3936-3944. doi: 10.1007/s00284-021-02654-y. Epub 2021 Sep 14.

DOI:10.1007/s00284-021-02654-y
PMID:34522977
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8439540/
Abstract

Our study attempted to explore the mechanism underlying the role of LuxR family transcriptional regulator abaR in biofilm formation by Acinetobacter baumannii. The abaR gene was knocked out in ATCC 17978 strain using homologous recombination method. The growth curve and biofilm formation were measured in the wild type and abaR gene knockdown strains. Transcriptome sequencing was performed in the wild type and abaR gene knockdown strains following 8 h of culture. The growth curve in the abaR gene knockdown strain was similar to that of the wild-type strain. Biofilm formation significantly declined in the abaR gene knockdown strain at 8 and 48 h after culture. A total of 137 differentially expressed genes (DEGs) were obtained including 20 downregulated DEGs and 117 upregulated DEGs. Genes with differential expression were closely related to viral procapsid maturation (GO:0046797), acetoin catabolism (GO:0045150), carbon metabolism (ko01200), and the glycolysis/gluconeogenesis (ko00010)-related pathways. The results of the eight verified expression DEGs were consistent with the results predicted by bioinformatics. AbaR gene knockdown significantly affected biofilm formation by A. baumannii ATCC 17978 strain. The glycolysis/gluconeogenesis pathways were significantly dysregulated and induced by abaR gene knockdown in A. baumannii.

摘要

本研究试图探讨鲍曼不动杆菌 LuxR 家族转录调节因子 abaR 在生物膜形成中作用的机制。采用同源重组的方法敲除 ATCC 17978 株中的 abaR 基因。在野生型和 abaR 基因敲除株中测量生长曲线和生物膜形成。在培养 8 小时后，在野生型和 abaR 基因敲除株中进行转录组测序。abaR 基因敲除株的生长曲线与野生型菌株相似。在培养 8 和 48 小时后，abaR 基因敲除株的生物膜形成显著下降。共获得 137 个差异表达基因（DEGs），包括 20 个下调的 DEGs 和 117 个上调的 DEGs。差异表达的基因与病毒衣壳成熟（GO：0046797）、乙酰丁醇分解代谢（GO：0045150）、碳代谢（ko01200）和糖酵解/糖异生（ko00010）相关途径密切相关。8 个验证表达的 DEGs 的结果与生物信息学预测的结果一致。abaR 基因敲除显著影响了 ATCC 17978 株鲍曼不动杆菌的生物膜形成。abaR 基因敲除在鲍曼不动杆菌中显著调节和诱导糖酵解/糖异生途径。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/8439540/dcd4a262daf6/284_2021_2654_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/8439540/574bbc1d1476/284_2021_2654_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/8439540/5b9a4cafef2d/284_2021_2654_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/8439540/b532797b696e/284_2021_2654_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/8439540/71758efc8385/284_2021_2654_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/8439540/fc1706a74761/284_2021_2654_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/8439540/dcd4a262daf6/284_2021_2654_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/8439540/574bbc1d1476/284_2021_2654_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/8439540/5b9a4cafef2d/284_2021_2654_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/8439540/b532797b696e/284_2021_2654_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/8439540/71758efc8385/284_2021_2654_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/8439540/fc1706a74761/284_2021_2654_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6d4/8439540/dcd4a262daf6/284_2021_2654_Fig6_HTML.jpg

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