基于 N-酰基高丝氨酸内酯的群体感应对高负荷反应器中 ANAMMOX 颗粒特性的长期影响。
Long-term effects of N-acyl-homoserine lactone-based quorum sensing on the characteristics of ANAMMOX granules in high-loaded reactors.
机构信息
The College of Architecture and Civil Engineering, The Key Laboratory of Beijing for Water Quality Science & Water Environment Recovery Engineering, Beijing University of Technology, Beijing, 100124, China.
The College of Architecture and Civil Engineering, The Key Laboratory of Beijing for Water Quality Science & Water Environment Recovery Engineering, Beijing University of Technology, Beijing, 100124, China.
出版信息
Chemosphere. 2019 Mar;218:632-642. doi: 10.1016/j.chemosphere.2018.11.170. Epub 2018 Nov 26.
Laboratory experiments were carried out to determine the long-term effects of N-acyl-homoserine lactone (AHL)-based quorum sensing on the characteristics of ANAMMOX granules in high-loaded reactors. Results clearly showed that adding 30 mg L N-octanoyl-DL-homoserinelactone (C-HSL) at the initial stage (1-40 d) of the experiment had long-term positive effects on the settleability of granules and controlled the sludge floatation effectively. C-HSL decreased the content of bound extracellular polymeric substances (B-EPS) and the ratio of protein to carbohydrate (PN/PS) by 17% and 48%, respectively and increased the relative hydrophobicity (RH) of the granules by 28%. The results of batch tests indicated that C-HSL significantly reduced the content of loosely-bound EPS (LB-EPS) in the B-EPS, which was responsible for variations in granule settleability and stability. Thus, the settleability of the granules was improved significantly due to addition of C-HSL, contributing to operational stability and the high TN removal efficiency of the reactor. On day 150, when the nitrogen loading rates of all reactors were 13.4 kg TN m d, the nitrogen removal rate and nitrogen removal efficiency of the reactor with C-HSL (R3) were up to 11.2 kg TN m d and 88%, respectively. N-hexanoyl-DL-homoserine lactone (C-HSL) improved activity of the granules, while N-dodecanoyl-DL-homoserine lactone (C-HSL) had no effect on the characteristics of the granules. The long-term effects of C-HSL on the settleability of granules may be attributed to sustainable release of endogenous signals induced by exogenous signal.
实验室实验旨在确定基于 N-酰基高丝氨酸内酯（AHL）的群体感应对高负荷反应器中 ANAMMOX 颗粒特性的长期影响。结果清楚地表明，在实验的初始阶段（1-40 天）添加 30mg/L 的 N-辛酰基-DL-高丝氨酸内酯（C-HSL）对颗粒的沉降性能具有长期的积极影响，并有效地控制了污泥浮渣。C-HSL 使颗粒的结合态胞外聚合物（B-EPS）含量和蛋白质与碳水化合物的比例（PN/PS）分别降低了 17%和 48%，并使颗粒的相对疏水性（RH）增加了 28%。批处理测试的结果表明，C-HSL 显著降低了 B-EPS 中松散结合的 EPS（LB-EPS）的含量，这是导致颗粒沉降性能和稳定性变化的原因。因此，由于添加了 C-HSL，颗粒的沉降性能得到了显著改善，从而提高了反应器的运行稳定性和高 TN 去除效率。在第 150 天，当所有反应器的氮负荷率均为 13.4kgTNm d 时，添加 C-HSL（R3）的反应器的氮去除率和氮去除效率分别高达 11.2kgTNm d 和 88%。N-己酰基-DL-高丝氨酸内酯（C-HSL）提高了颗粒的活性，而 N-十二酰基-DL-高丝氨酸内酯（C-HSL）对颗粒的特性没有影响。C-HSL 对颗粒沉降性能的长期影响可能归因于外源性信号诱导的内源性信号的持续释放。
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