Lasater T M, Lefebvre R C, Assaf A R, Saritelli A L, Carleton R A
Department of Community Health, Brown University, Providence, Rhode Island.
Am J Prev Med. 1987 Nov-Dec;3(6):311-6.
Rapid and reliable techniques for the measurement of blood cholesterol are a necessary first step in implementing effective mass screening efforts to reduce the risks of cardiovascular disease. This paper reports preliminary data gathered on the Kodak Ektachem DT60, one of the first of a new generation of dry chemistry analyzers to appear on the market. The results of the first study demonstrated high reproducibility of the methodology (r = .98, p less than .0001) but showed an average 25 mg/dL underestimate of total blood cholesterol as compared with a standardized lipid laboratory. Changes in the manufacturer's calibration protocol in the second study with two other DT60s reduced this bias on serum samples to -18 mg/dL. A third study using fresh blood samples found a difference of 3-11 mg/dL between the two DT60s and the laboratory. A significant difference between the two analyzers was also found. Alternatives are available to enable those who use this instrument to correct for the observed bias. Despite some problems, several other advantages of the technology lend support for its expanded use in public health interventions.
快速可靠的血液胆固醇测量技术是开展有效大规模筛查以降低心血管疾病风险的必要第一步。本文报告了在柯达Ektachem DT60上收集的初步数据，这是新一代干式化学分析仪中最早出现在市场上的产品之一。第一项研究结果表明该方法具有高度可重复性（r = 0.98，p小于0.0001），但与标准化脂质实验室相比，总血胆固醇平均低估25mg/dL。在第二项研究中，使用另外两台DT60并更改制造商的校准方案后，血清样本的这种偏差降至-18mg/dL。第三项使用新鲜血液样本的研究发现，两台DT60与实验室之间的差异为3 - 11mg/dL。还发现两台分析仪之间存在显著差异。有一些方法可供使用该仪器的人校正观察到的偏差。尽管存在一些问题，但该技术的其他几个优点支持其在公共卫生干预中的更广泛应用。
