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构巢曲霉线粒体DNA的限制性内切酶切割图谱

Restriction endonuclease cleavage map of mitochondrial DNA from Aspergillus nidulans.

作者信息

Stepień P P, Bernard U, Cooke H J, Küntzel H

出版信息

Nucleic Acids Res. 1978 Feb;5(2):317-30. doi: 10.1093/nar/5.2.317.

DOI:10.1093/nar/5.2.317
PMID:345242
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC341986/
Abstract

Mitochondrial DNA of the ascomycete fungus Aspergillus nidulans, a circular molecule of 31 500 base pairs, is cleaved by restriction endonucleases Eco R I, Hind II, Hind III and Bgl II into 3, 7, 9 and 5 fragments, respectively. The relative positions of the cleavage sites could be mapped by analysis of fragments obtained by double enzyme digestions of whole DNA and by complete and partial redigestion of isolated restriction fragments.

摘要

子囊菌构巢曲霉的线粒体DNA是一个由31500个碱基对组成的环状分子，分别被限制性内切酶Eco R I、Hind II、Hind III和Bgl II切割成3、7、9和5个片段。通过对全DNA进行双酶切以及对分离的限制性片段进行完全和部分再消化所获得的片段进行分析，可以绘制出切割位点的相对位置。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c72d/341986/51f9a65d79dc/nar00463-0025-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c72d/341986/ccce88ceef82/nar00463-0024-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c72d/341986/51f9a65d79dc/nar00463-0025-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c72d/341986/ccce88ceef82/nar00463-0024-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c72d/341986/51f9a65d79dc/nar00463-0025-a.jpg

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引用本文的文献

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本文引用的文献

1
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Biochim Biophys Acta. 1966 Jan 11;113(1):51-6. doi: 10.1016/s0926-6593(66)80120-0.
2
Nuclear and extranuclear inheritance of oligomycin resistance in Aspergillus nidulans.构巢曲霉中寡霉素抗性的核遗传和核外遗传
Mol Gen Genet. 1973 Nov 12;126(3):201-16. doi: 10.1007/BF00267531.
3
Cytoplasmic inheritance of a cold-sensitive mutant in Aspergillus nidulans.构巢曲霉中一个冷敏感突变体的细胞质遗传
丝状子囊菌多育赤霉线粒体 DNA：线粒体染色体的特征及限制性内切酶多态性的群体遗传学。
Curr Genet. 1984 Oct;8(8):621-8. doi: 10.1007/BF00395708.
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Nucleotide sequence of 5S ribosomal RNA from Aspergillus nidulans and Neurospora crassa.来自构巢曲霉和粗糙脉孢菌的5S核糖体RNA的核苷酸序列。
Nucleic Acids Res. 1981 Mar 25;9(6):1445-50. doi: 10.1093/nar/9.6.1445.
5
Nucleotide sequence of the Aspergillus nidulans mitochondrial gene coding for the small ribosomal subunit RNA: homology to E. coli 16S rRNA.构巢曲霉线粒体基因编码小核糖体亚基RNA的核苷酸序列：与大肠杆菌16S rRNA的同源性
Nucleic Acids Res. 1981 Nov 11;9(21):5689-96. doi: 10.1093/nar/9.21.5689.
6
The cloning of Aspergillus nidulans mitochondrial DNA in Escherichia coli on plasmid pBR322.构巢曲霉线粒体DNA在大肠杆菌中于质粒pBR322上的克隆。
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7
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8
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Unidirectional gene conversion associated with two insertions in neurospora crassa mitochondrial DNA.与粗糙脉孢菌线粒体DNA中的两个插入相关的单向基因转换。
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5
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Proc Natl Acad Sci U S A. 1974 Jul;71(7):2823-7. doi: 10.1073/pnas.71.7.2823.
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Translation products in vitro of mitochondrial messenger RNA from Neurospora crassa.粗糙脉孢菌线粒体信使核糖核酸的体外翻译产物
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