构巢曲霉线粒体DNA的限制性内切酶切割图谱
Restriction endonuclease cleavage map of mitochondrial DNA from Aspergillus nidulans.
作者信息
Stepień P P, Bernard U, Cooke H J, Küntzel H
出版信息
Nucleic Acids Res. 1978 Feb;5(2):317-30. doi: 10.1093/nar/5.2.317.
Abstract
Mitochondrial DNA of the ascomycete fungus Aspergillus nidulans, a circular molecule of 31 500 base pairs, is cleaved by restriction endonucleases Eco R I, Hind II, Hind III and Bgl II into 3, 7, 9 and 5 fragments, respectively. The relative positions of the cleavage sites could be mapped by analysis of fragments obtained by double enzyme digestions of whole DNA and by complete and partial redigestion of isolated restriction fragments.
摘要
子囊菌构巢曲霉的线粒体DNA是一个由31500个碱基对组成的环状分子，分别被限制性内切酶Eco R I、Hind II、Hind III和Bgl II切割成3、7、9和5个片段。通过对全DNA进行双酶切以及对分离的限制性片段进行完全和部分再消化所获得的片段进行分析，可以绘制出切割位点的相对位置。
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