大鼠肝脏线粒体DNA的限制性内切酶切割图谱。

The restriction endonuclease cleavage map of rat liver mitochondrial DNA.

作者信息

Kroon A M, Bakker H, Holtrop M, Terpstra P

出版信息

Biochim Biophys Acta. 1977 Jan 3;474(1):61-8. doi: 10.1016/0005-2787(77)90214-3.

Abstract

Mitochondrial DNA from rat liver contains six sites for cleavage by the restriction endonucleases Hind III and EcoRI. A large stretch of DNA, comprising about 40% of the mitochondrial genome is not cleaved by either of the enzymes; eight cleavage sites are located on a DNA stretch of 35% of the genome length suggestive of an unequal distribution of the A - T baspairs over the molecule. The number of Hind III and Eco R I fragments is much higher than reported for other mammalian mitochondrial DNAs up to now.

摘要

大鼠肝脏的线粒体DNA含有六个可被限制性内切酶Hind III和EcoRI切割的位点。一大段DNA，约占线粒体基因组的40%，不会被这两种酶中的任何一种切割；八个切割位点位于占基因组长度35%的一段DNA上，这表明A - T碱基对在分子上分布不均。Hind III和Eco R I片段的数量比迄今为止报道的其他哺乳动物线粒体DNA的数量要多得多。