Huang Wanqiu, Wang Danni, Yao Yu-Feng
Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Department of Infectious Diseases, Shanghai Ruijin Hospital, Shanghai 200025, China.
Microb Cell. 2021 Aug 4;8(9):208-222. doi: 10.15698/mic2021.09.759. eCollection 2021 Sep 6.
Infections are highly orchestrated and dynamic processes, which involve both pathogen and host. Transcriptional profiling at the single-cell level enables the analysis of cell diversity, heterogeneity of the immune response, and detailed molecular mechanisms underlying infectious diseases caused by bacteria, viruses, fungi, and parasites. Herein, we highlight recent remarkable advances in single-cell RNA sequencing (scRNA-seq) technologies and their applications in the investigation of host-pathogen interactions, current challenges and potential prospects for disease treatment are discussed as well. We propose that with the aid of scRNA-seq, the mechanism of infectious diseases will be further revealed thus inspiring the development of novel interventions and therapies.
感染是高度有序且动态的过程，涉及病原体和宿主双方。单细胞水平的转录谱分析能够分析细胞多样性、免疫反应的异质性以及由细菌、病毒、真菌和寄生虫引起的传染病背后的详细分子机制。在此，我们重点介绍单细胞RNA测序（scRNA-seq）技术的最新显著进展及其在宿主-病原体相互作用研究中的应用，同时也讨论了当前疾病治疗面临的挑战和潜在前景。我们认为，借助scRNA-seq，传染病的机制将得到进一步揭示，从而推动新型干预措施和疗法的发展。
