Department of Biology, Faculty of Science, Research Institute for Grape and Raisins, Malayer University, Malayer, Iran.
Department of Chemistry, Faculty of Science, Research Institute for Grape and Raisins, Malayer University, Malayer, Iran.
J Cancer Res Ther. 2021 Jul-Sep;17(4):875-878. doi: 10.4103/jcrt.JCRT_766_19.
Previous studies have suggested that consuming fruit and vegetable can lower the risk of several cancers, including breast, colorectal, and lung cancers.
The present study aims to investigate the in vitro anticancer effects of Shahani and Asgari grape seed extract (GSE) grown in Malayer City of Iran on HL-60 cancer. However, to the best of the author's knowledge, it is the first time in this study that the antiproliferative effect of Shahani and Asgari GSE is compared.
Shahani and Asgari GSE Was extraction white method of Liquid/liquid extraction with ethyl acetate. Then assessing cytotoxic activities of Shahani and Asgari GSE on the HL-60 cells was tested using MTT assay.
The results show that compared with the control group, seed extract of both Shahani and Asgari at the various concentrations (25, 50, 100, and 200 μg/ml) had a significantly inhibitory effect on HL-60 cell proliferation that was dose dependent. However, Shahani GSE at different concentrations (50, 100, and 200 μg/ml) indicated a significantly higher inhibitory effect compared to Asgari GSE. In addition, GSE can induce cell cycle arrest at G0/G1 cells. Furthermore, GSE of Asgari and Shahani remarkably increased the induction of HL-60 cell apoptosis depending on its dose. However, at the concentration of 200 μg/ml, GSE induced cell necrosis rather than apoptosis.
Seed extract of both Shahani and Asgari at the various concentrations had a significantly inhibitory effect on HL-60 cell proliferation that was dose dependent.
先前的研究表明，摄入水果和蔬菜可以降低多种癌症的风险，包括乳腺癌、结直肠癌和肺癌。
本研究旨在探讨伊朗马勒耶市种植的沙哈尼和阿斯加里葡萄籽油（GSE）在体外对 HL-60 癌症的抗癌作用。然而，据作者所知，这是首次比较沙哈尼和阿斯加里 GSE 的抗增殖作用。
沙哈尼和阿斯加里 GSE 通过乙酸乙酯的液/液萃取方法提取。然后使用 MTT 测定法测试 Shahani 和 Asgari GSE 对 HL-60 细胞的细胞毒性活性。
结果表明，与对照组相比，沙哈尼和阿斯加里种子提取物在不同浓度（25、50、100 和 200μg/ml）下对 HL-60 细胞增殖均有明显的抑制作用，呈剂量依赖性。然而，Shahani GSE 在不同浓度（50、100 和 200μg/ml）下对 HL-60 细胞的抑制作用明显高于 Asgari GSE。此外，GSE 可以诱导细胞周期停滞在 G0/G1 期。此外，GSE 可显著增加 HL-60 细胞凋亡的诱导，且与剂量相关。然而，在 200μg/ml 的浓度下，GSE 诱导细胞坏死而不是凋亡。
沙哈尼和阿斯加里的种子提取物在不同浓度下对 HL-60 细胞增殖均有明显的抑制作用，呈剂量依赖性。
