末端炔烃的氘代实现了类似炔基标记生物分子的同时活细胞 Raman 成像。
Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules.
机构信息
Synthetic Organic Chemistry Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
RIKEN Center for Sustainable Resource Science, 2-1, Hirosawa, Wako, Saitama 351-0198, Japan.
出版信息
Org Biomol Chem. 2021 Oct 6;19(38):8232-8236. doi: 10.1039/d1ob01479j.
PMID:34528645
Abstract
Alkynes were employed as tags to observe small molecules in cells by Raman microscopy. Herein, simple deuteration was found to shift the vibrational frequency of the alkyne by 135 cm. Two-color Raman imaging of D-alkynes and H-alkynes made it possible to distinguish between and observe similar small molecules in live cells.
摘要
炔烃被用作拉曼显微镜观察细胞中小分子的标记。本文发现，简单的氘代会使炔烃的振动频率移动 135cm。D-炔烃和 H-炔烃的双色拉曼成像使得在活细胞中区分和观察类似的小分子成为可能。
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