Synthetic Organic Chemistry Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan; Catalysis and Integrated Research Group, RIKEN Center for Sustainable Resource Science, 2-1, Hirosawa, Wako, Saitama, 351-0198, Japan.
Synthetic Organic Chemistry Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan; Catalysis and Integrated Research Group, RIKEN Center for Sustainable Resource Science, 2-1, Hirosawa, Wako, Saitama, 351-0198, Japan.
Curr Opin Chem Biol. 2022 Oct;70:102181. doi: 10.1016/j.cbpa.2022.102181. Epub 2022 Jul 2.
Raman microscopy has been used to deduce information about the distributions of endogenous biomolecules without exogenous labeling. Several functional groups, such as alkynes (CC), nitriles (CN), and carbon-deuterium (C-D) bonds, have been employed in recent years as Raman tags to detect target molecules in cells. In this article, we review some recent advances in applications using deuterated fatty acids for lipid analysis, such as investigation of tumor-selective cytotoxicity of γ-linolenic acid (GLA), simultaneous two-color imaging of stearate and oleate using deuterated and protonated alkynes, Raman hyperspectral imaging, and analyses of the physical properties of lipids through spectral unmixing of the C-D vibrational frequencies. In addition, we review some advanced methods for observing intracellular metabolic activities, such as de novo lipogenesis from deuterium-labeled precursors.
拉曼显微镜已被用于推导内源性生物分子的分布信息，而无需外源性标记。近年来，一些官能团，如炔烃（CC）、腈（CN）和碳氘（C-D）键，已被用作拉曼标记物来检测细胞中的靶分子。在本文中，我们综述了近年来使用氘代脂肪酸进行脂质分析的一些应用进展，例如研究 γ-亚麻酸（GLA）的肿瘤选择性细胞毒性，使用氘代和质子化炔烃同时进行硬脂酸盐和油酸盐的双色成像，拉曼高光谱成像，以及通过 C-D 振动频率的光谱解混分析脂质的物理性质。此外，我们还综述了一些观察细胞内代谢活性的先进方法，例如从氘标记前体从头合成脂肪。
