Zhu Chen-Yuan, Zhu Yi-Hao, Zhou Hua-Ping, Xu Yuan-Yuan, Gao Jian, Zhang Ye-Wang
School of Pharmacy, Jiangsu University, Zhenjiang, People's Republic of China.
College of Petroleum and Chemical Engineering, Beibu Gulf University, Qinzhou, People's Republic of China.
Prep Biochem Biotechnol. 2022;52(5):590-597. doi: 10.1080/10826068.2021.1975299. Epub 2021 Sep 16.
A novel arabitol dehydrogenase (ArDH) gene was cloned from a bacterium named and expressed heterologously in . The purified ArDH exhibited the maximal activity in pH 9.5 Tris-HCl buffer at 40 °C, showed and of 1.2 mg/mL and 9.1 U/mg, respectively. The ArDH was used to produce the L-xylulose and coupled with the NADH oxidase (Nox) for the regeneration of NAD. In further optimization, a high conversion of 84.6% in 8 hours was achieved under the optimal conditions: 20 mM of xylitol, 100 µM NAD in pH 9.0 Tris-HCl buffer at 30 °C. The results indicated the coupling system with cofactor regeneration provides a promising approach for L-xylulose production from xylitol.
从一种名为 的细菌中克隆出一个新的阿拉伯糖醇脱氢酶（ArDH）基因，并在 中进行了异源表达。纯化后的ArDH在40°C的pH 9.5 Tris-HCl缓冲液中表现出最大活性，其Km和Vmax分别为1.2 mg/mL和9.1 U/mg。该ArDH用于生产L-木酮糖，并与NADH氧化酶（Nox）偶联以实现NAD的再生。在进一步优化中，在最佳条件下：20 mM木糖醇、100 µM NAD、pH 9.0 Tris-HCl缓冲液、30°C，8小时内实现了84.6%的高转化率。结果表明，具有辅因子再生的偶联系统为从木糖醇生产L-木酮糖提供了一种有前景的方法。
