Tsumura Kampo Research Laboratories, Tsumura & Co., Ibaraki, Japan.
Biosci Biotechnol Biochem. 2021 Oct 21;85(11):2274-2280. doi: 10.1093/bbb/zbab162.
Dementia is exacerbated by loss of appetite and amotivation, and recent studies have indicated that ninjin'yoeito improves anorexia and amotivation. Previous studies suggest that ninjin'yoeito inhibits dopamine-metabolizing enzymes and enhances dopamine signaling. However, whether ninjin'yoeito increases dopamine content in living cells remains unclear. Here, PC12 cells were used to examine whether ninjin'yoeito affects the dopamine metabolic pathway. Dopamine content significantly increased 3 h after treatment ninjin'yoeito extract. Concomitantly, the levels of 3-methoxytyramine and 3,4-dihydroxyphenylacetic acid were significantly reduced. The effects of components of ninjin'yoeito on the dopamine metabolic pathway were also assessed. Treatment with onjisaponin B, nobiletin, and schisandrin, and the ingredients of Polygalae Radix, Citri Unshiu Pericarpium, and Schisandrae Fructus increased dopamine content and decreased its metabolite content in the culture media. Our findings suggest that ninjin'yoeito improves anorexia and amotivation by inhibiting metabolic enzyme and increasing the dopamine content in cells.
痴呆症会因食欲不振和缺乏动力而加重，最近的研究表明，人参汤能改善厌食症和缺乏动力。先前的研究表明，人参汤能抑制多巴胺代谢酶并增强多巴胺信号。然而，人参汤是否能增加活细胞中的多巴胺含量仍不清楚。在这里，我们使用 PC12 细胞来研究人参汤是否会影响多巴胺代谢途径。人参汤提取物处理 3 小时后，多巴胺含量显著增加。同时，3-甲氧基酪胺和 3,4-二羟基苯乙酸的水平也显著降低。还评估了人参汤成分对多巴胺代谢途径的影响。荭草苷 B、川陈皮素和五味子醇甲以及远志、陈皮和五味子的成分能增加多巴胺含量，并降低培养基中多巴胺代谢产物的含量。我们的研究结果表明，人参汤通过抑制代谢酶和增加细胞内多巴胺含量来改善厌食症和缺乏动力。
