Faculty of dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Department of Endodontics, Faculty of dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Aust Endod J. 2022 Aug;48(2):274-282. doi: 10.1111/aej.12565. Epub 2021 Sep 16.
The aim of this study was to assess the effect of antimicrobial photodynamic therapy (aPDT) with curcumin and riboflavin on three-week Enterococcus faecalis biofilm. At first the 15-mm root canals of 65 single rooted extracted human teeth (including maxillary incisors, mandibular and maxillary canines and mandibular premolars) were separated from the crown and were prepared with ProTaper instruments. After autoclave sterilisation, samples were inoculated with E. faecalis suspension, and incubated for three weeks. After ensuring biofilm formation by scanning electron microscopy (SEM) in two teeth, the remaining 63 teeth were randomly divided into seven groups (n = 9): aPDT + curcumin, aPDT + riboflavin, LED, curcumin, riboflavin, 5.25% NaOCl (positive control) and no intervention (negative control). For light source a LED unit with 390-480 nm wavelength (peak of 460 nm), power density of 1000 ± 100 mW cm and mean energy density of 60 J cm was used. The roots were horizontally sectioned into coronal, middle and apical thirds each with 5 mm thicknesses. Dentin chips with equal weight (1 ± 0.005 g) were collected from the root canal walls with Gates Glidden drills and were transferred into microtubes containing 1 mL of sterile saline and vortexed for 30 s. Next, 10 µL of the contents of each tube was serially diluted and eventually, 10 µL of each solution was cultured on BHI agar. The number of colony-forming units was determined. Data were analysed using the Kruskal-Wallis and Friedman tests. The colony reduction was not significantly different between NaOCl and either riboflavin + LED or Curcumin + LED. The 5.25% NaOCl group showed maximum reduction in colony count, compared with the negative control (P = 0.00). Groups with aPDT with Curcumin + LED (P = 0.005), and with riboflavin + LED (P = 0.011) showed significant reduction in colony count in all three canal thirds (P < 0.05) without any difference with one another. With significant reduction of E. faecalis colony count, aPDT with Curcumin and riboflavin can serve as an adjunct to routine root canal disinfection method.
本研究旨在评估姜黄素和核黄素的抗菌光动力疗法（aPDT）对三周粪肠球菌生物膜的影响。首先，将 65 颗单根人离体牙（包括上颌切牙、下颌和上颌尖牙以及下颌前磨牙）的 15-mm 根管从牙冠中分离出来，并使用 ProTaper 器械进行预备。经过高压灭菌消毒后，样本接种粪肠球菌悬液，孵育三周。通过扫描电子显微镜（SEM）在两颗牙齿上确认生物膜形成后，将其余 63 颗牙齿随机分为七组（n=9）：aPDT+姜黄素、aPDT+核黄素、LED、姜黄素、核黄素、5.25%次氯酸钠（阳性对照）和无干预（阴性对照）。光源为波长 390-480nm（峰值 460nm）、功率密度 1000±100mW/cm2 和平均能量密度 60J/cm2 的 LED 装置。将牙根水平切成冠、中、根尖三部分，每部分 5mm 厚。用 Gates Glidden 钻头从根管壁上采集重量相等（1±0.005g）的牙本质屑，转移到含有 1mL 无菌生理盐水的微量管中，涡旋 30s。然后，从每个管的内容物中取 10μL进行连续稀释，最终从每个溶液中取 10μL在 BHI 琼脂上培养。确定菌落形成单位的数量。使用 Kruskal-Wallis 和 Friedman 检验分析数据。次氯酸钠与核黄素+LED 或姜黄素+LED 之间的菌落减少没有显著差异。与阴性对照组相比，5.25%次氯酸钠组的菌落计数减少最多（P=0.00）。与 Curcumin+LED（P=0.005）和 Riboflavin+LED（P=0.011）组的 aPDT 组在所有三个根管段的菌落计数均有显著减少（P<0.05），彼此之间没有差异。粪肠球菌菌落计数显著减少，姜黄素和核黄素的 aPDT 可作为常规根管消毒方法的辅助手段。
