Maltarollo Thalya Fernanda Horsth, Dos Santos Paulo Henrique, Banci Henrique Augusto, Bachega Mariana de Oliveira, de Oliveira Beatriz Melare, Duarte Marco Hungaro Antonio, Queiroz Índia Olinta de Azevedo, Amaral Rodrigo Rodrigues, Cintra Luciano Angelo Tavares, Strazzi-Sahyon Henrico Badaoui, Sivieri-Araujo Gustavo
Department of Preventive and Restorative Dentistry, Discipline of Endodontics, Araçatuba School of Dentistry, São Paulo State University - UNESP, Araçatuba, SP, Brazil.
Restorative Dentistry Area, Faculty of Dentistry, University of Toronto, Toronto, ON, Canada.
Lasers Med Sci. 2025 Jan 28;40(1):51. doi: 10.1007/s10103-025-04302-4.
Investigate the impact of antimicrobial photodynamic therapy (aPDT) using different photosensitizers (PSs) such as indocyanine green (IG), curcumin (CC), and methylene blue (MB), with or without intracanal application of calcium hydroxide (CH), on the push-out bond strength of glass-fiber posts (GFPs) to intraradicular dentin, the chemical composition of the root substrate, and the sealing of the adhesive interface across different thirds of intraradicular dentin. A total of 112 bovine teeth underwent biomechanical preparation and were divided into eight experimental groups (n = 14 each): Negative control with deionized water; positive control with deionized water + CH; IG group with indocyanine green and infrared laser; IG + CH group; CC group with curcumin and blue LED; CC + CH group; MB group with methylene blue and red laser; and MB + CH group. The push-out bond strength was measured using a universal testing machine (n = 8), and scanning electron microscopy characterized the fracture patterns. Energy dispersive spectroscopy (n = 3) analyzed the chemical composition of the dentin substrate, while fluorescence confocal microscopy (n = 3) assessed the adhesive interface sealing between the resin cement and root dentin. Data were analyzed using two-way repeated measures ANOVA and the Tukey test for push-out bond strength and chemical composition comparison, with the Kruskal-Wallis and Dunn's tests (α = 0.05) for adhesive interface sealing. Significant bond strength differences were noted across root thirds and experimental groups (P < .05), with the IG + CH group showing the highest cervical bond strength and the IG group the lowest. Apical bond strength was highest in the CC group but lower in the NC and PC groups. Mixed failures predominated, except in the MB + CH group, where adhesive failures prevailed. Elemental composition varied among groups treated with different PSs and CH (P < .05), but interface quality, tag formation, and penetration depth showed no significant differences (P > .05). Laser-activated 500 mg/L CC combined with CH emerged as a clinically relevant option for root canal decontamination before GFPs luting. aPDT with different PSs and root canal depth influenced the push-out bond strength of GFPs and the chemical composition of root dentin. Curcumin-mediated aPDT at 500 mg/L proved effective, enhancing bond strength and sealing while maintaining consistent dentin composition across depths.
研究使用不同光敏剂（PSs）如吲哚菁绿（IG）、姜黄素（CC）和亚甲蓝（MB），以及有无根管内应用氢氧化钙（CH）的抗菌光动力疗法（aPDT）对玻璃纤维桩（GFPs）与根管内牙本质的推出粘结强度、牙根基底的化学成分以及根管内牙本质不同三分之一处粘结界面封闭性的影响。总共112颗牛牙经过生物力学预备，并分为八个实验组（每组n = 14）：用去离子水的阴性对照组；用去离子水 + CH的阳性对照组；用吲哚菁绿和红外激光的IG组；IG + CH组；用姜黄素和蓝色发光二极管的CC组；CC + CH组；用亚甲蓝和红色激光的MB组；以及MB + CH组。使用万能试验机测量推出粘结强度（n = 8），扫描电子显微镜表征断裂模式。能量色散光谱分析牙本质基底的化学成分（n = 3），而荧光共聚焦显微镜评估树脂水门汀与牙根牙本质之间的粘结界面封闭性（n = 3）。使用双向重复测量方差分析和Tukey检验分析数据，用于推出粘结强度和化学成分比较，对于粘结界面封闭性使用Kruskal-Wallis和Dunn检验（α = 0.05）。在牙根不同三分之一处和实验组之间观察到显著的粘结强度差异（P < 0.05），IG + CH组显示出最高的颈部粘结强度，IG组最低。根尖粘结强度在CC组中最高，但在阴性对照组和阳性对照组中较低。除了MB + CH组以粘结失败为主外，混合性失败占主导。用不同PSs和CH处理的组之间元素组成有所不同（P < 0.05），但界面质量、标签形成和渗透深度无显著差异（P > 0.05）。激光激活的500 mg/L CC联合CH成为GFPs粘结前根管去污的一种临床相关选择。用不同PSs和根管深度的aPDT影响GFPs的推出粘结强度和牙根牙本质的化学成分。500 mg/L姜黄素介导的aPDT被证明是有效的，增强了粘结强度和封闭性，同时在不同深度保持牙本质成分一致。
