Ziegler S F, Marth J D, Lewis D B, Perlmutter R M
Mol Cell Biol. 1987 Jun;7(6):2276-85. doi: 10.1128/mcb.7.6.2276-2285.1987.
Protein-tyrosine kinases are implicated in the control of cell growth by virtue of their frequent appearance as products of retroviral oncogenes and as components of growth factor receptors. Here we report the characterization of a novel human protein-tyrosine kinase gene (hck) that is primarily expressed in hematopoietic cells, particularly granulocytes. The hck gene encodes a 505-residue polypeptide that is closely related to pp56lck, a lymphocyte-specific protein-tyrosine kinase. The exon breakpoints of the hck gene, partially defined by using murine genomic clones, demonstrate that hck is a member of the src gene family and has been subjected to strong selection pressure during mammalian evolution. High-level expression of hck transcripts in granulocytes is especially provocative since these cells are terminally differentiated and typically survive in vivo for only a few hours. Thus the hck gene, like other members of the src gene family, appears to function primarily in cells with little growth potential.
蛋白质酪氨酸激酶常作为逆转录病毒癌基因的产物以及生长因子受体的组成部分，与细胞生长的调控有关。在此，我们报告了一个新的人类蛋白质酪氨酸激酶基因（hck）的特征，该基因主要在造血细胞中表达，尤其是粒细胞。hck基因编码一个由505个氨基酸残基组成的多肽，它与淋巴细胞特异性蛋白质酪氨酸激酶pp56lck密切相关。通过使用小鼠基因组克隆部分确定的hck基因外显子断点表明，hck是src基因家族的成员，并且在哺乳动物进化过程中受到了强烈的选择压力。hck转录本在粒细胞中的高水平表达尤其令人关注，因为这些细胞是终末分化的，并且在体内通常仅存活数小时。因此，hck基因与src基因家族的其他成员一样，似乎主要在生长潜力较小的细胞中发挥作用。
