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麝香酮通过调控miRNA - 145抑制胃癌。

Muscone suppresses gastric cancer via regulation of miRNA-145.

作者信息

Gao Feng, Yan Shihai, Sun Zheng, Wang Jia

机构信息

Department of Clinical Laboratory Jiangsu Province Hospital of Chinese Medicine Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing China.

出版信息

Food Sci Nutr. 2021 Jul 26;9(9):4711-4721. doi: 10.1002/fsn3.2269. eCollection 2021 Sep.

DOI:10.1002/fsn3.2269
PMID:34531985
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8441313/
Abstract

This study aims to determine the effects and mechanism of action of muscone on the biological activity of the gastric cancer cell lines SGC-7901 and MGC-803 (proliferation, apoptosis, invasion, and migration) in vitro. An optimal muscone concentration was determined using MTT and cell apoptosis tests. The SGC-7901 and MGC-803 cells were divided into five groups: normal control, muscone, miRNA, muscone + miRNA, and muscone + miRNA inhibitor. Cell proliferation rate, apoptosis rate, cell cycle phase distribution, number of invading cells, and wound healing rate were compared among the five groups using MTT, flow cytometry, transwell, and wound healing assays. Relative expression levels of the proteins PI3K, AKT, P21, c-Myc, MMP-2, and MMP-9 were measured by Western blot. Compared with the control group, the groups treated with muscone and miRNA showed significantly lower cell proliferation rate, number of invading cells, and wound healing rate ( < .05 for all), but significantly higher rates of cell apoptosis rate and numbers of cells in the G1 phase ( < .05 for all). These groups also showed significantly lower expression of the proteins PI3K, AKT, c-Myc, MMP-2, and MMP-9 but significantly increased expression of the protein P21 ( < .05). Transfecting muscone-treated SGC-7901 and MGC-803 cells with miRNA-145 inhibitor resulted in a significant recovery of biological activity ( < .05). Muscone suppresses the biological activity of SGC-7901 and MGC-803 gastric cancer cells in vitro via regulation of miRNA-145.

摘要

本研究旨在确定麝香酮对胃癌细胞系SGC - 7901和MGC - 803体外生物学活性（增殖、凋亡、侵袭和迁移）的影响及作用机制。使用MTT和细胞凋亡试验确定了最佳麝香酮浓度。将SGC - 7901和MGC - 803细胞分为五组：正常对照、麝香酮、miRNA、麝香酮 + miRNA以及麝香酮 + miRNA抑制剂组。使用MTT、流式细胞术、Transwell和伤口愈合试验比较五组细胞的增殖率、凋亡率、细胞周期阶段分布、侵袭细胞数量和伤口愈合率。通过蛋白质免疫印迹法检测PI3K、AKT、P21、c - Myc、MMP - 2和MMP - 9蛋白的相对表达水平。与对照组相比，麝香酮组和miRNA组细胞增殖率、侵袭细胞数量和伤口愈合率显著降低（均P < 0.05），但细胞凋亡率和G1期细胞数量显著升高（均P < 0.05）。这些组PI3K、AKT、c - Myc、MMP - 2和MMP - 9蛋白表达也显著降低，但P21蛋白表达显著升高（P < 0.05）。用miRNA - 145抑制剂转染经麝香酮处理的SGC - 7901和MGC - 803细胞后，生物学活性显著恢复（P < 0.05）。麝香酮通过调控miRNA - 145体外抑制SGC - 7901和MGC - 803胃癌细胞的生物学活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/468b/8441313/bf8bf5421566/FSN3-9-4711-g007.jpg
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