Department of Immunology and School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Viral Immunol. 2021 Dec;34(10):708-713. doi: 10.1089/vim.2021.0061. Epub 2021 Sep 17.
The coronavirus infectious disease 2019 (COVID-19), which is initiated by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has imposed critical challenges to global health. Understanding the kinetic of SARS-CoV-2-specific IgM and IgG responses in different subsets of COVID-19 patients is crucial to get insight into the humoral immune response elicited against the virus. We investigated IgM and IgG responses against SARS-CoV-2 nucleocapsid (N) and receptor-binding domain (RBD) of spike protein in two groups of recovered and deceased COVID-19 patients. The levels of IgM and IgG specific to N and RBD proteins were detected by ELISA. N- and RBD-specific IgM was higher in deceased patients in comparison with recovered patients, while there was no significant difference in N- and RBD-specific IgG between the two groups. A significant correlation was observed between IgG and IgM titers against RBD and N, in both groups of patients. These results argue against impaired antibody response in deceased COVID-19 patients.
2019 年冠状病毒病（COVID-19）由严重急性呼吸系统综合征冠状病毒 2（SARS-CoV-2）引发，对全球健康造成了重大挑战。了解 COVID-19 患者不同亚群中 SARS-CoV-2 特异性 IgM 和 IgG 反应的动力学对于深入了解针对该病毒产生的体液免疫反应至关重要。我们研究了两组已康复和已死亡 COVID-19 患者中针对 SARS-CoV-2 核衣壳（N）和刺突蛋白受体结合域（RBD）的 IgM 和 IgG 反应。通过 ELISA 检测针对 N 和 RBD 蛋白的 IgM 和 IgG 特异性。与已康复患者相比，死亡患者的 N 和 RBD 特异性 IgM 水平更高，而两组患者的 N 和 RBD 特异性 IgG 之间没有显著差异。在两组患者中，均观察到 IgG 和 IgM 针对 RBD 和 N 的滴度之间存在显著相关性。这些结果表明死亡 COVID-19 患者的抗体反应并未受损。
