Ferreira J L, Hamdy M K, Herd Z L, McCay S G, Zapatka F A
FDA, Atlanta, Georgia 30309.
Mol Cell Probes. 1987 Dec;1(4):337-45. doi: 10.1016/0890-8508(87)90015-6.
Hybridomas producing monoclonal antibodies against type A Hall strain Clostridium botulinum toxin were generated by fusing mouse myeloma cell line P3-X63-Ag8.653 with spleen cells of Balb/c mice immunized with C. botulinum type A toxoid. The monoclonal antibody from one hybridoma, identified as No. 424, was selected from 61 others for its high antibody titre. This monoclonal antibody was used in a double-sandwich enzyme-linked immunosorbent assay (ELISA) system to detect type A toxin in culture fluids and in foods. The monoclonal antibody did not react with either C. botulinum toxin types B, C, D, E and F or with other clostridial species tested. This particular monoclonal antibody (No. 424) did not neutralize type A toxin in the mouse bioassay procedure but detected approximately 10 mouse lethal doses of type A toxin/ml culture fluid. Monoclonal antibody and rabbit antitoxin to type A C. botulinum toxin were useful in a double-sandwich ELISA for the rapid and specific detection of type A toxic fluids in culture and in food samples.
通过将小鼠骨髓瘤细胞系P3-X63-Ag8.653与用A型肉毒杆菌类毒素免疫的Balb/c小鼠的脾细胞融合，产生了针对A型霍尔株肉毒杆菌毒素的单克隆抗体杂交瘤。从61个其他杂交瘤中选出一种来自编号为424的杂交瘤的单克隆抗体，因其抗体滴度高。该单克隆抗体用于双夹心酶联免疫吸附测定（ELISA）系统，以检测培养液和食品中的A型毒素。该单克隆抗体与B、C、D、E和F型肉毒杆菌毒素或所检测的其他梭菌属均无反应。这种特定的单克隆抗体（编号424）在小鼠生物测定程序中不能中和A型毒素，但能检测出约10个小鼠致死剂量的A型毒素/毫升培养液。单克隆抗体和兔抗A型肉毒杆菌毒素抗毒素可用于双夹心ELISA，以快速、特异性地检测培养液和食品样品中的A型有毒液体。
