利用原子力显微镜测量死后时间和年龄对小鼠晶状体弹性的影响。
Measuring the effects of postmortem time and age on mouse lens elasticity using atomic force microscopy.
机构信息
Biomedical Atomic Force Microscopy Laboratory, Department of Biomedical Engineering, University of Miami College of Engineering, Miami, FL, USA.
Biomedical Atomic Force Microscopy Laboratory, Department of Biomedical Engineering, University of Miami College of Engineering, Miami, FL, USA; Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
出版信息
Exp Eye Res. 2021 Nov;212:108768. doi: 10.1016/j.exer.2021.108768. Epub 2021 Sep 14.
The mouse lens is frequently used both in vivo and ex vivo in ophthalmic research to model conditions affecting the human lens, such as presbyopia. The mouse lens has a delicate structure which is prone to damage and biomechanical changes both before and after extraction from the whole globe. When not properly controlled for, these changes can confound the biomechanical analysis of mouse lenses. In this study, atomic force microscopy microindentation was used to assess changes in the Young's Modulus of Elasticity of the mouse lens as a function of mouse age and postmortem time. Old mouse lenses measured immediately postmortem were significantly stiffer than young mouse lenses (p = 0.028). However, after 18 h of incubation, there was no measurable difference in lens stiffness between old and young mouse lenses (p = 0.997). This demonstrates the need for careful experimental control in experiments using the mouse lens, especially regarding postmortem time.
鼠标镜头在眼科研究中经常被用于体内和体外，以模拟影响人眼晶状体的条件，如远视。鼠标镜头结构精细，在从整个眼球中提取前后都容易受到损伤和生物力学变化的影响。如果不加以适当控制，这些变化可能会混淆对鼠标镜头的生物力学分析。在这项研究中，原子力显微镜微压痕被用于评估鼠标晶状体杨氏弹性模量随鼠标年龄和死后时间的变化。死后立即测量的老年鼠标晶状体明显比年轻鼠标晶状体硬（p = 0.028）。然而，在孵育 18 小时后，老年和年轻鼠标晶状体之间的晶状体硬度没有可测量的差异（p = 0.997）。这表明在使用鼠标晶状体的实验中需要仔细的实验控制，特别是关于死后时间。
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