Guangdong Pharmaceutical University, Guangzhou, 510000, Guangdong, China; The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, 510000, Guangdong, China.
The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, 510000, Guangdong, China.
Microvasc Res. 2022 Jan;139:104257. doi: 10.1016/j.mvr.2021.104257. Epub 2021 Sep 15.
Amiodarone is the first choice for the treatment of arrhythmia, but it is easy to cause extravasation during infusion, after extravasation, it often cause skin injury. The healing of skin injury induced by amiodarone is an inflammatory process. Chrysin, a natural flavonoid, has been investigated to have anti-inflammatory and antioxidant effects. It was reported that chrysin can promote wound healing. So this study aims to investigate the effect of chrysin on amiodarone extravasation-induced skin injury model in rats.
The rat model of skin extravasation injury was established by subcutaneous injection of 0.5 mL of amiodarone. After successful modeling, the rats were randomly assigned to the five groups: control group, 10% DMSO group, and low-dose, medium-dose, and high-dose chrysin groups (10, 20 and 40 mg/mL). The extravasation injury model was given locally on the same day for seven days. On day 0, 3, 7 and 10 of administration, the lesion's image were taken to calculate the area, and the tissue of the lesion were collected for H&E staining. Then, the level of IL-6 and TNF-α were measured by ELISA, and the protein expression level of bFGF in the wound tissue were detected by immunohistochemical staining.
It was found that chrysin groups (20 and 40 mg/mL) compared to contronl group and 10% DMSO solvent group significantly decreased area injury, IL-6 and TNF-α(P < 0.05) on day 3, 7, 10. On the other hand, the chrysin group (40 mg/mL) compared to contronl group and 10% DMSO group significantly increase bFGF(P < 0.05) on day 3, 7.
Chrysin were effective in reducing injury area, reducing inflammation, and promoting the secretion of bFGF, it can promote the healing of skin injury induced by amiodarone extravasation in rats. These findings provide a good theoretical and experimental basis for the prevention and treatment of amiodarone extravasation-induced skin injury, and provide evidence for finding potential healing agents for the prevention and treatment of amiodarone and other corrosive extravasation-induced injuries from the molecular and cytological levels, thus solving the clinical problems.
胺碘酮是治疗心律失常的首选药物，但在输注过程中容易发生外渗，外渗后常导致皮肤损伤。胺碘酮引起的皮肤损伤的愈合是一个炎症过程。白杨素是一种天然黄酮类化合物，已被研究具有抗炎和抗氧化作用。据报道，白杨素可以促进伤口愈合。因此，本研究旨在探讨白杨素对大鼠胺碘酮外渗性皮肤损伤模型的影响。
通过皮下注射 0.5 mL 胺碘酮建立大鼠皮肤外渗损伤模型。建模成功后，将大鼠随机分为对照组、10% DMSO 组和低、中、高剂量白杨素组（10、20 和 40 mg/mL）。在同一天局部给予外渗损伤模型，连续给药 7 天。在给药第 0、3、7 和 10 天，拍摄病变图像计算面积，并收集病变组织进行 H&E 染色。然后，通过 ELISA 测量 IL-6 和 TNF-α的水平，通过免疫组化染色检测伤口组织中 bFGF 的蛋白表达水平。
发现与对照组和 10% DMSO 溶剂组相比，白杨素组（20 和 40 mg/mL）在第 3、7、10 天显著减少了损伤面积、IL-6 和 TNF-α（P<0.05）。另一方面，与对照组和 10% DMSO 组相比，白杨素组（40 mg/mL）在第 3、7 天显著增加了 bFGF（P<0.05）。
白杨素有减轻损伤面积、减轻炎症和促进 bFGF 分泌的作用，能促进大鼠胺碘酮外渗性皮肤损伤的愈合。这些发现为预防和治疗胺碘酮外渗性皮肤损伤提供了良好的理论和实验依据，并为从分子和细胞学水平寻找预防和治疗胺碘酮和其他腐蚀性外渗损伤的潜在愈合剂提供了证据，从而解决了临床问题。
